| 产品名称： | pC21/3c |
|---|---|
| 商品货号： | TS157017 |
| Designations： | pC21/3c |
| GenBank Number： | M13936 |
| Species： | Homo sapiens, human |
| Depositors： | PC White |
| Applications： | Hybridizes with CYP21A (pseudogene) and CYP21B. Detects bands of 3.2 kb (A) and 3.7 kb (B) in TaqI digests which can be used to detect deletions and duplications. |
| Vector： | Construct size (kb): 5.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.099999904632568 Tissue: fetal adrenal gland Gene product: cytochrome P450, subfamily XXI (steroid 21-hydroxylase, congenital adrenal hyperplasia) CYP21 Alleles: A2, B2, A2, B2, A1, B1, A1, B1, C2, C2, C1, C1 |
| Insert Size (kb)： | 2.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 2.2; XhoI--3.0, 2.3; PstI--1.95, 1.2, 0.53, 0.47 + others; HindIII--5.2; ClaI--5.2. This encodes the complete cDNA except for about 30 bp at the 5 end. The poly(A) signal is 21 bp upstream of the poly(A) sequence (42 bp). Hybridizes with CYP21A (pseudogene) and CYP21B. Detects bands of 3.2 kb (A) and 3.7 kb (B) in TaqI digests which can be used to detect deletions and duplications. Detects bands of 10 kb (A) and 12 kb (B) in BglII digests. |
| References： | White PC, et al. Two genes encoding steroid 21-hydroxylase are located near the genes encoding the fourth component of complement in man. Proc. Natl. Acad. Sci. USA 82: 1089-1093, 1985. PubMed: 2983330 White PC, et al. Structure of human steroid 21-hydroxylase genes. Proc. Natl. Acad. Sci. USA 83: 5111-5115, 1986. PubMed: 3487786 Werkmeister JW, et al. Frequent deletion and duplication of the steroid 21-hydroxylase genes. Am. J. Hum. Genet. 39: 461-469, 1986. PubMed: 3490178 Amor M, et al. Mutation in the CYP21B gene (Ile-172-->Asn) causes steroid 21-hydroxylase deficiency. Proc. Natl. Acad. Sci. USA 85: 1600-1604, 1988. PubMed: 3257825 |
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| 产品名称： | pC21/3c |
|---|---|
| 商品货号： | TS157017 |
| Designations： | pC21/3c |
| GenBank Number： | M13936 |
| Species： | Homo sapiens, human |
| Depositors： | PC White |
| Applications： | Hybridizes with CYP21A (pseudogene) and CYP21B. Detects bands of 3.2 kb (A) and 3.7 kb (B) in TaqI digests which can be used to detect deletions and duplications. |
| Vector： | Construct size (kb): 5.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.099999904632568 Tissue: fetal adrenal gland Gene product: cytochrome P450, subfamily XXI (steroid 21-hydroxylase, congenital adrenal hyperplasia) CYP21 Alleles: A2, B2, A2, B2, A1, B1, A1, B1, C2, C2, C1, C1 |
| Insert Size (kb)： | 2.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 2.2; XhoI--3.0, 2.3; PstI--1.95, 1.2, 0.53, 0.47 + others; HindIII--5.2; ClaI--5.2. This encodes the complete cDNA except for about 30 bp at the 5 end. The poly(A) signal is 21 bp upstream of the poly(A) sequence (42 bp). Hybridizes with CYP21A (pseudogene) and CYP21B. Detects bands of 3.2 kb (A) and 3.7 kb (B) in TaqI digests which can be used to detect deletions and duplications. Detects bands of 10 kb (A) and 12 kb (B) in BglII digests. |
| References： | White PC, et al. Two genes encoding steroid 21-hydroxylase are located near the genes encoding the fourth component of complement in man. Proc. Natl. Acad. Sci. USA 82: 1089-1093, 1985. PubMed: 2983330 White PC, et al. Structure of human steroid 21-hydroxylase genes. Proc. Natl. Acad. Sci. USA 83: 5111-5115, 1986. PubMed: 3487786 Werkmeister JW, et al. Frequent deletion and duplication of the steroid 21-hydroxylase genes. Am. J. Hum. Genet. 39: 461-469, 1986. PubMed: 3490178 Amor M, et al. Mutation in the CYP21B gene (Ile-172-->Asn) causes steroid 21-hydroxylase deficiency. Proc. Natl. Acad. Sci. USA 85: 1600-1604, 1988. PubMed: 3257825 |
