pVL-RabGGTbeta-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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pVL-RabGGTbeta
pVL-RabGGTbeta
规格:
货期:
编号:TS157752
品牌:Testobio
产品名称: pVL-RabGGTbeta
商品货号: TS157752
Designations: pVL-RabGGTbeta
GenBank Number:

L10416

Species: Rattus norvegicus, rat
Depositors: MC Seabra, S Armstrong
Applications:
in suitable host, produces protein rab geranylgeranyl transferase beta Rab geranylgeranyl transferase, beta subunit
Vector:
Construct size (kb): 11.0
Insert:
DNA: cDNA
Insert lengths(kb): 1.299999952316284
Tissue: brain
Gene product: rab geranylgeranyl transferase beta(Rab geranylgeranyl transferase, beta subunit) GNGNT2B*
Target Gene: rab geranylgeranyl transferase beta
Insert Size (kb): 1.300
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--9.6, 1.3; BamHI--9.8, 0.9; XhoI--7.7, 2.3.
The insert contains the following restriction sites (approximate kb from the 5 end): XhoI--0.07; BglII--0.59,1.14; BamHI--0.87; BclI--1.29.
When pVL-RabGGTalpha and pVL-RabGGTbeta (ATCC 87154, 87155) are used to generate recombinant baculoviruses and infect Sf9 cells, the active dimer is produced.
References:

Cremers FP, et al. REP-2, a Rab escort protein encoded by the choroideremia-like gene. J. Biol. Chem. 269: 2111-2117, 1994. PubMed: 8294464

Luckow VA, Summers MD. Signals Important for high-level expression of foreign genes in Autographa californica Nuclear Polyhedrosis Virus expression vectors. Virology 167: 56-71, 1988. PubMed: 3142147

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pVL-RabGGTbeta

  • 货号: TS157752
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: pVL-RabGGTbeta
商品货号: TS157752
Designations: pVL-RabGGTbeta
GenBank Number:

L10416

Species: Rattus norvegicus, rat
Depositors: MC Seabra, S Armstrong
Applications:
in suitable host, produces protein rab geranylgeranyl transferase beta Rab geranylgeranyl transferase, beta subunit
Vector:
Construct size (kb): 11.0
Insert:
DNA: cDNA
Insert lengths(kb): 1.299999952316284
Tissue: brain
Gene product: rab geranylgeranyl transferase beta(Rab geranylgeranyl transferase, beta subunit) GNGNT2B*
Target Gene: rab geranylgeranyl transferase beta
Insert Size (kb): 1.300
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--9.6, 1.3; BamHI--9.8, 0.9; XhoI--7.7, 2.3.
The insert contains the following restriction sites (approximate kb from the 5 end): XhoI--0.07; BglII--0.59,1.14; BamHI--0.87; BclI--1.29.
When pVL-RabGGTalpha and pVL-RabGGTbeta (ATCC 87154, 87155) are used to generate recombinant baculoviruses and infect Sf9 cells, the active dimer is produced.
References:

Cremers FP, et al. REP-2, a Rab escort protein encoded by the choroideremia-like gene. J. Biol. Chem. 269: 2111-2117, 1994. PubMed: 8294464

Luckow VA, Summers MD. Signals Important for high-level expression of foreign genes in Autographa californica Nuclear Polyhedrosis Virus expression vectors. Virology 167: 56-71, 1988. PubMed: 3142147

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