| 产品名称： | pSCH131 |
|---|---|
| 商品货号： | TS158238 |
| Designations： | pSCH131 |
| Species： | Unidentified bacterium |
| Depositors： | KJ Shaw |
| Insert： | DNA: Synthetic Insert lengths(kb): 0.296999990940094 Gene product: aminoglycoside acetyltransferase (6) type I aac(6)-In |
| Insert Size (kb)： | 0.297 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.2; BamHI--3.2; HindIII--3.2; BssHII--2.7, 0.45. A single gel purification of the PCR generated probe is necessary since flanking regions will co-amplify with the gene specific sequence. Failure to do so often results in high backgrounds and false positives with clinical E. coli strains. A hybridization probe may be generated using the following vector specific PCR primers: modified T3 = 5-CCCCTCACTAAAGGGAACAAAAGCTG-3 and modified T7 = 5-CGCGTAATACGACTCACTATAGGGCGAA-3. |
| References： | Shaw KJ, et alThe application of molecular techniques for the study of aminoglycoside resistanceIn: Shaw KJ, et alMethods in molecular medicine: molecular approaches for the diagnosis and investigation of bacterial diseasesTotowa, NJHumana Presssubmitted, 1996 |
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| 产品名称： | pSCH131 |
|---|---|
| 商品货号： | TS158238 |
| Designations： | pSCH131 |
| Species： | Unidentified bacterium |
| Depositors： | KJ Shaw |
| Insert： | DNA: Synthetic Insert lengths(kb): 0.296999990940094 Gene product: aminoglycoside acetyltransferase (6) type I aac(6)-In |
| Insert Size (kb)： | 0.297 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.2; BamHI--3.2; HindIII--3.2; BssHII--2.7, 0.45. A single gel purification of the PCR generated probe is necessary since flanking regions will co-amplify with the gene specific sequence. Failure to do so often results in high backgrounds and false positives with clinical E. coli strains. A hybridization probe may be generated using the following vector specific PCR primers: modified T3 = 5-CCCCTCACTAAAGGGAACAAAAGCTG-3 and modified T7 = 5-CGCGTAATACGACTCACTATAGGGCGAA-3. |
| References： | Shaw KJ, et alThe application of molecular techniques for the study of aminoglycoside resistanceIn: Shaw KJ, et alMethods in molecular medicine: molecular approaches for the diagnosis and investigation of bacterial diseasesTotowa, NJHumana Presssubmitted, 1996 |
