human Id-1b [phId-1b]-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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human Id-1b [phId-1b]
human Id-1b [phId-1b]
规格:
货期:
编号:TS158447
品牌:Testobio
产品名称: human Id-1b phId-1b
商品货号: TS158447
Designations: human Id-1b phId-1b
GenBank Number:

S78825

Species: Homo sapiens, human
Applications:
contains sequence inhibitor of DNA binding 1, dominant negative helix-loop-helix protein Inhibitor of DNA binding 1, dominant negative, Id1
Vector:
Construct size (kb): 4.099999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI NotI XmaIII SacII BstXI SacI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ID1
Gene name: inhibitor of DNA binding 1, dominant negative
helix-loop-helix protein
Contains complete coding sequence?: Y
Chromosome: 20; Localization: 20 q11
Tissue: U251 glioblastoma cell line
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.145
Cross references: DNA Seq. Acc.: S78825
Insert lengths(kb): 1.144999980926514
Tissue: U251 glioblastoma cell line
Gene product: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein( Inhibitor of DNA binding 1, dominant negative, Id1) ID1
Target Gene: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
Insert Size (kb): 1.145
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.2; BglI--2.2, 1.3, 0.7; KpnI--3.5, 0.65; PvuI--2.1, 1.1, 1.0, 0.1; PvuII--2.5, 1.4, 0.3.
Insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.42; KpnI--0.54; PvuI--0.70, 0.83; PvuII--0.05; SmaI--0.36; SphI--0.70.
There is an erroneous single extra nucleotide "t" at position 1091 in GenBank record S78225.
The clones human Id-1a (ATCC 99522/3) and human Id-1b (ATCC 99524/5) represent alternatively spliced products of the same gene.
References:

Zhu W, et al. Id gene expression during development and molecular cloning of the human Id-1 gene. Brain Res. Mol. Brain Res. 30: 312-326, 1995. PubMed: 7637581

Mark A Israel, personal communication

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human Id-1b [phId-1b]

  • 货号: TS158447
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: human Id-1b phId-1b
商品货号: TS158447
Designations: human Id-1b phId-1b
GenBank Number:

S78825

Species: Homo sapiens, human
Applications:
contains sequence inhibitor of DNA binding 1, dominant negative helix-loop-helix protein Inhibitor of DNA binding 1, dominant negative, Id1
Vector:
Construct size (kb): 4.099999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI NotI XmaIII SacII BstXI SacI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ID1
Gene name: inhibitor of DNA binding 1, dominant negative
helix-loop-helix protein
Contains complete coding sequence?: Y
Chromosome: 20; Localization: 20 q11
Tissue: U251 glioblastoma cell line
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.145
Cross references: DNA Seq. Acc.: S78825
Insert lengths(kb): 1.144999980926514
Tissue: U251 glioblastoma cell line
Gene product: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein( Inhibitor of DNA binding 1, dominant negative, Id1) ID1
Target Gene: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
Insert Size (kb): 1.145
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.2; BglI--2.2, 1.3, 0.7; KpnI--3.5, 0.65; PvuI--2.1, 1.1, 1.0, 0.1; PvuII--2.5, 1.4, 0.3.
Insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.42; KpnI--0.54; PvuI--0.70, 0.83; PvuII--0.05; SmaI--0.36; SphI--0.70.
There is an erroneous single extra nucleotide "t" at position 1091 in GenBank record S78225.
The clones human Id-1a (ATCC 99522/3) and human Id-1b (ATCC 99524/5) represent alternatively spliced products of the same gene.
References:

Zhu W, et al. Id gene expression during development and molecular cloning of the human Id-1 gene. Brain Res. Mol. Brain Res. 30: 312-326, 1995. PubMed: 7637581

Mark A Israel, personal communication

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