pCD.2
pCD.2
规格:
货期:
编号:TS159362
品牌:Testobio
| 产品名称： | pCD.2 |
|---|---|
| 商品货号： | TS159362 |
| Designations： | pCD.2 |
| Depositors： | PC Zambryski |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli DH5 |
| Vector Information： | Size (kb): 6.2199997901916500 DESCRIPTION OF VECTOR: Intact vector size: 6.220 Type of vector: plasmid Cloning sites: NdeI NcoI AvrII BglII BclI BamHI SmaI SalI PstI Polylinker sites: NdeI NcoI AvrII BglII BclI BamHI SmaI SalI PstI Construction: pRS314 Features (with orientation and position when available): replicon: ColE1, 1-362 coding sequence: bla, replicon: ARS4, 2066-2442 centromere: CEN6, 2028-2053 marker(s): TRP1, ->, 2976-3650 promoter: PADH, 4437-5165 MCS: NdeI..PstI, ->, 5661-5730 ribosome-binding site: GAL4, ->, 5166-5607 transcription terminator: ADC1 transcriptional terminator (TADH), 5748-5938 Vector: pCD.2 (plasmid) Promoters: Promoter PADH Construction: pRS314 Marker(s):crbR Construct size (kb): 6.21999979019165 Features: marker(s): TRP1 promoter: PADH replicon: ARS4 replicon: ColE1 MCS: NdeI..PstI ribosome-binding site: GAL4 transcription terminator: ADC1 transcriptional terminator (TADH) centromere: CEN6 coding sequence: bla |
| Applications： | cloning vector shuttle vector vector component of two-hybrid screening system |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.2; PstI--6.2; HindIII--4.5, 1.7; NdeI--6.2. Useful in two hybrid screening in yeast. A CEN-based plasmid with the Gal4 DNA binding domain. It has lower copy number than 2 micron based plasmids. Contains unique rstriction sites (BglII, BclI and BamHI) compatible with 5 GATC overhangs in all three reading frames. |
| Media： | ATCC® Medium 1637: LB medium (ATCC medium 1065) with 100 mcg/ml carbenicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Durfee T, et al. New tools for protein linkage mapping and general two-hybrid screening. Yeast 15: 1761-1768, 1999. PubMed: 10590464 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
pCD.2
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | pCD.2 |
|---|---|
| 商品货号： | TS159362 |
| Designations： | pCD.2 |
| Depositors： | PC Zambryski |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli DH5 |
| Vector Information： | Size (kb): 6.2199997901916500 DESCRIPTION OF VECTOR: Intact vector size: 6.220 Type of vector: plasmid Cloning sites: NdeI NcoI AvrII BglII BclI BamHI SmaI SalI PstI Polylinker sites: NdeI NcoI AvrII BglII BclI BamHI SmaI SalI PstI Construction: pRS314 Features (with orientation and position when available): replicon: ColE1, 1-362 coding sequence: bla, replicon: ARS4, 2066-2442 centromere: CEN6, 2028-2053 marker(s): TRP1, ->, 2976-3650 promoter: PADH, 4437-5165 MCS: NdeI..PstI, ->, 5661-5730 ribosome-binding site: GAL4, ->, 5166-5607 transcription terminator: ADC1 transcriptional terminator (TADH), 5748-5938 Vector: pCD.2 (plasmid) Promoters: Promoter PADH Construction: pRS314 Marker(s):crbR Construct size (kb): 6.21999979019165 Features: marker(s): TRP1 promoter: PADH replicon: ARS4 replicon: ColE1 MCS: NdeI..PstI ribosome-binding site: GAL4 transcription terminator: ADC1 transcriptional terminator (TADH) centromere: CEN6 coding sequence: bla |
| Applications： | cloning vector shuttle vector vector component of two-hybrid screening system |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.2; PstI--6.2; HindIII--4.5, 1.7; NdeI--6.2. Useful in two hybrid screening in yeast. A CEN-based plasmid with the Gal4 DNA binding domain. It has lower copy number than 2 micron based plasmids. Contains unique rstriction sites (BglII, BclI and BamHI) compatible with 5 GATC overhangs in all three reading frames. |
| Media： | ATCC® Medium 1637: LB medium (ATCC medium 1065) with 100 mcg/ml carbenicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Durfee T, et al. New tools for protein linkage mapping and general two-hybrid screening. Yeast 15: 1761-1768, 1999. PubMed: 10590464 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
