5HT1Dbeta-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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5HT1Dbeta
5HT1Dbeta
规格:
货期:
编号:TS159657
品牌:Testobio
产品名称: 5HT1Dbeta
商品货号: TS159657
Designations: 5HT1Dbeta
GenBank Number:

L09732

Species: Homo sapiens, human
Depositors: MJ Bienkowski, SA Veldman
Applications:
in another host, produces protein 5-hydroxytryptamine (serotonin) receptor 1D beta
Vector:
Construct size (kb): 8.710000038146973
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: p3CLNeo
Intact vector size: 7.440
Type of vector: plasmid
Vector end: HindIII
Vector end: SalI
Cloning sites: HindIII SalI
Polylinker sites: HindIII SalI
Construction: p3CL, neoR
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, G418
promoter: CMV
replicon: pMB1, SV40
terminator: bGH polyadenylation
Cross references:
Insert:
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: HTR1DB*
Gene name: 5-hydroxytryptamine (serotonin) receptor 1D beta
Contains complete coding sequence?: Y
Tissue: placenta, male
Type of DNA: genomic
Insert 5 end: HindIII
Insert 3 end: SalI
Insert size (kb): 1.27
Cross references: DNA Seq. Acc.: L09732
Insert lengths(kb): 1.269999980926514
Tissue: placenta, male
Gene product: 5-hydroxytryptamine (serotonin) receptor 1D beta HTR1DB*
Target Gene: 5-hydroxytryptamine (serotonin) receptor 1D beta
Insert Size (kb): 1.270
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): BglI--3.2, 1.9, 1.8, 1.1, 0.7; HindIII/SalI--7.4, 1.3; PstI--6.5, 1.3, 0.9; PvuII--3.5, 2.3, 1.1, 0.78, 0.58, 0.44; SmaI--5.7, 3.0; EcoRI--6.0, 3.0; EcoRI/XhoI--6.0, 2.35, 0.65.
The insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.30; NcoI--0.03; PstI--0.13; PvuII--0.80; SmaI--0.82.
Includes 22 bp of 5 untranslated sequence, the complete coding region, and 64 bp of 3 untranslated sequence.
The order of the major features of the plasmid is: ampR - EcoRI - SV40 ori - SV40 promoter - XhoI - neoR - SV40 polyadenylation - EcoRI - CMV promoter - HindIII - insert - SalI - bGH polyadenylation - PvuI.
References:

Veldman SA, Bienkowski MJ. Cloning and pharmacological characterization of a novel human 5-hydroxytryptamine1D receptor subtype. Mol. Pharmacol. 42: 439-444, 1992. PubMed: 1328844

Sarah A Veldman, personal communication

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5HT1Dbeta

  • 货号: TS159657
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: 5HT1Dbeta
商品货号: TS159657
Designations: 5HT1Dbeta
GenBank Number:

L09732

Species: Homo sapiens, human
Depositors: MJ Bienkowski, SA Veldman
Applications:
in another host, produces protein 5-hydroxytryptamine (serotonin) receptor 1D beta
Vector:
Construct size (kb): 8.710000038146973
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: p3CLNeo
Intact vector size: 7.440
Type of vector: plasmid
Vector end: HindIII
Vector end: SalI
Cloning sites: HindIII SalI
Polylinker sites: HindIII SalI
Construction: p3CL, neoR
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, G418
promoter: CMV
replicon: pMB1, SV40
terminator: bGH polyadenylation
Cross references:
Insert:
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: HTR1DB*
Gene name: 5-hydroxytryptamine (serotonin) receptor 1D beta
Contains complete coding sequence?: Y
Tissue: placenta, male
Type of DNA: genomic
Insert 5 end: HindIII
Insert 3 end: SalI
Insert size (kb): 1.27
Cross references: DNA Seq. Acc.: L09732
Insert lengths(kb): 1.269999980926514
Tissue: placenta, male
Gene product: 5-hydroxytryptamine (serotonin) receptor 1D beta HTR1DB*
Target Gene: 5-hydroxytryptamine (serotonin) receptor 1D beta
Insert Size (kb): 1.270
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): BglI--3.2, 1.9, 1.8, 1.1, 0.7; HindIII/SalI--7.4, 1.3; PstI--6.5, 1.3, 0.9; PvuII--3.5, 2.3, 1.1, 0.78, 0.58, 0.44; SmaI--5.7, 3.0; EcoRI--6.0, 3.0; EcoRI/XhoI--6.0, 2.35, 0.65.
The insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.30; NcoI--0.03; PstI--0.13; PvuII--0.80; SmaI--0.82.
Includes 22 bp of 5 untranslated sequence, the complete coding region, and 64 bp of 3 untranslated sequence.
The order of the major features of the plasmid is: ampR - EcoRI - SV40 ori - SV40 promoter - XhoI - neoR - SV40 polyadenylation - EcoRI - CMV promoter - HindIII - insert - SalI - bGH polyadenylation - PvuI.
References:

Veldman SA, Bienkowski MJ. Cloning and pharmacological characterization of a novel human 5-hydroxytryptamine1D receptor subtype. Mol. Pharmacol. 42: 439-444, 1992. PubMed: 1328844

Sarah A Veldman, personal communication

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