Perkinsus marinus (Mackin et al.) Levine-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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Perkinsus marinus (Mackin et al.) Levine
Perkinsus marinus (Mackin et al.) Levine
规格:
货期:
编号:TS159963
品牌:Testobio
产品名称： Perkinsus marinus (Mackin et al.) Levine
商品货号： TS159963
Strain Designations： HCedar2
Biosafety Level： 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：
eastern oyster, Crassotrea virginica, Cedar Keys, FL, 1998
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic： Axenic
Type Strain： no
Medium： ATCC® Medium 1886: Perkinsus broth medium
Growth Conditions：
Temperature: 25°C
Cryopreservation：
  1. Harvest cells from several cultures which are in logarithmic to late stationary phase of growth.xa0 Vigorously agitate to suspend the cells.
  2. Aseptically transfer the cell suspension to 15 ml plastic centrifuge tubes.
  3. Centrifuge at 200 x g for 5 min.
  4. While cells are centrifuging, prepare a 20% solution of DMSO in ATCC Medium 1886.
  5. Remove the supernatant and pool the cell pellets into a final volume of 4.5 ml.
  6. Combine the cell suspension with an equal volume of 20% DMSO cryoprotectant solution (prepared in step 4) to yield a final concentration of 10% DMSO.
  7. Dispense in 0.5 ml aliquots to 1.0-2.0 ml Nunc vials (special plastic vials for cryopreservation).
  8. Place the vials in a controlled rate freezing unit.xa0 From room temperature cool at -1°C/min to -40°C.xa0 At -40°C, plunge ampules into liquid nitrogen.
  9. Store ampules in a liquid nitrogen refrigerator until needed.
  10. To establish a culture from the frozen state, place a frozen ampule in a 35°C water bath just enough to cover the frozen material.xa0 Allow the ampule to thaw completely (2-3 min).
  11. Immediately after thawing, aseptically remove the contents and transfer to a T-25 tissue culture flask containing 10 ml of fresh ATCC medium 1886.
  12. Screw the cap on tightly and incubate at 25°C.xa0 Observe the culture daily.xa0 Transfer the culture when many trophozoites are observed.xa0xa0xa0xa0xa0xa0xa0xa0xa0
Name of Depositor： D Bushek
Chain of Custody：
ATCC <-- D Bushek <-- K. Hudson
Year of Origin： 1998
References：

David Bushek, personal communication

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Perkinsus marinus (Mackin et al.) Levine

  • 货号: TS159963
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称： Perkinsus marinus (Mackin et al.) Levine
商品货号： TS159963
Strain Designations： HCedar2
Biosafety Level： 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：
eastern oyster, Crassotrea virginica, Cedar Keys, FL, 1998
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic： Axenic
Type Strain： no
Medium： ATCC® Medium 1886: Perkinsus broth medium
Growth Conditions：
Temperature: 25°C
Cryopreservation：
  1. Harvest cells from several cultures which are in logarithmic to late stationary phase of growth.xa0 Vigorously agitate to suspend the cells.
  2. Aseptically transfer the cell suspension to 15 ml plastic centrifuge tubes.
  3. Centrifuge at 200 x g for 5 min.
  4. While cells are centrifuging, prepare a 20% solution of DMSO in ATCC Medium 1886.
  5. Remove the supernatant and pool the cell pellets into a final volume of 4.5 ml.
  6. Combine the cell suspension with an equal volume of 20% DMSO cryoprotectant solution (prepared in step 4) to yield a final concentration of 10% DMSO.
  7. Dispense in 0.5 ml aliquots to 1.0-2.0 ml Nunc vials (special plastic vials for cryopreservation).
  8. Place the vials in a controlled rate freezing unit.xa0 From room temperature cool at -1°C/min to -40°C.xa0 At -40°C, plunge ampules into liquid nitrogen.
  9. Store ampules in a liquid nitrogen refrigerator until needed.
  10. To establish a culture from the frozen state, place a frozen ampule in a 35°C water bath just enough to cover the frozen material.xa0 Allow the ampule to thaw completely (2-3 min).
  11. Immediately after thawing, aseptically remove the contents and transfer to a T-25 tissue culture flask containing 10 ml of fresh ATCC medium 1886.
  12. Screw the cap on tightly and incubate at 25°C.xa0 Observe the culture daily.xa0 Transfer the culture when many trophozoites are observed.xa0xa0xa0xa0xa0xa0xa0xa0xa0
Name of Depositor： D Bushek
Chain of Custody：
ATCC <-- D Bushek <-- K. Hudson
Year of Origin： 1998
References：

David Bushek, personal communication

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