| 产品名称： | pFL59- |
|---|---|
| 商品货号： | TS160097 |
| Designations： | pFL59- |
| Depositors： | F Lacroute |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.8010001182556150 Vector: pFL59- (phagemid) Promoters: Promoter lac Construction: pUC19 Marker(s):TRP1,ampR Construct size (kb): 4.801000118255615 Features: insert detection: lacZ marker(s): ampR, TRP1 promoter: lac replicon: pMB1, f1, ARS-CEN6 centromere: CEN6 |
| Applications： | YC-type (centromeric) shuttle vector shuttle vector vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.8; BglII--4.0, 0.85; ClaI--4.0, 0.8. YC-type low copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. Can be used to produce ssDNA. The order of the major features in this plasmid is: EcoRI/MCS/HindIII - TRP1 - ARS-CEN6 - pMB1 ori - ampR - f1 ori. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470 Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589 Bach ML, et al. Evidence for transcriptional regulation of OMP-decarboxylase in yeast by hybridisation of mRNA to the yeast structural gene cloned in E. coli. Proc. Natl. Acad. Sci. USA 76: 386-390, 1979. PubMed: 370827 Dotto GP, et al. The functional origin of Bacteriophage f1 DNA replication. J. Mol. Biol. 172: 507-521, 1984. PubMed: 6607361 Panzeri L, Philippsen P. Centromeric DNA from chromosome VI in Saccharomyces cerevisiae. EMBO J. 1: 1605-1611, 1982. PubMed: 6327282 |
| Shipped： | freeze-dried |
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| 产品名称： | pFL59- |
|---|---|
| 商品货号： | TS160097 |
| Designations： | pFL59- |
| Depositors： | F Lacroute |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.8010001182556150 Vector: pFL59- (phagemid) Promoters: Promoter lac Construction: pUC19 Marker(s):TRP1,ampR Construct size (kb): 4.801000118255615 Features: insert detection: lacZ marker(s): ampR, TRP1 promoter: lac replicon: pMB1, f1, ARS-CEN6 centromere: CEN6 |
| Applications： | YC-type (centromeric) shuttle vector shuttle vector vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.8; BglII--4.0, 0.85; ClaI--4.0, 0.8. YC-type low copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. Can be used to produce ssDNA. The order of the major features in this plasmid is: EcoRI/MCS/HindIII - TRP1 - ARS-CEN6 - pMB1 ori - ampR - f1 ori. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470 Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589 Bach ML, et al. Evidence for transcriptional regulation of OMP-decarboxylase in yeast by hybridisation of mRNA to the yeast structural gene cloned in E. coli. Proc. Natl. Acad. Sci. USA 76: 386-390, 1979. PubMed: 370827 Dotto GP, et al. The functional origin of Bacteriophage f1 DNA replication. J. Mol. Biol. 172: 507-521, 1984. PubMed: 6607361 Panzeri L, Philippsen P. Centromeric DNA from chromosome VI in Saccharomyces cerevisiae. EMBO J. 1: 1605-1611, 1982. PubMed: 6327282 |
| Shipped： | freeze-dried |
