HFBCB30-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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HFBCB30
HFBCB30
规格:
货期:
编号:TS160146
品牌:Testobio
产品名称: HFBCB30
商品货号: TS160146
Designations: HFBCB30
GenBank Number:

M77889

Species: Homo sapiens, human
Vector:
Construct size (kb): 5.099999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MAP4
Gene name: microtubule-associated protein 4
Contains complete coding sequence?: U
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.1
Cross references: DNA Seq. Acc.: M77889; EST: EST01473; Primer: M13 Forward;
THC id: THC102192
Insert lengths(kb): 2.099999904632568
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: microtubule-associated protein 4 MAP4
Insert Size (kb): 2.100
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.8, 0.3; PstI--3.0, 0.75, 0.54, 0.47, 0.1; HindIII--5.1.
EST01473 matches GenBank accession HUMMAP4 with 97.59% identity over the sequence range: 1-250.
References:

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

J C Venter, personal communication

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HFBCB30

  • 货号: TS160146
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: HFBCB30
商品货号: TS160146
Designations: HFBCB30
GenBank Number:

M77889

Species: Homo sapiens, human
Vector:
Construct size (kb): 5.099999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MAP4
Gene name: microtubule-associated protein 4
Contains complete coding sequence?: U
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.1
Cross references: DNA Seq. Acc.: M77889; EST: EST01473; Primer: M13 Forward;
THC id: THC102192
Insert lengths(kb): 2.099999904632568
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: microtubule-associated protein 4 MAP4
Insert Size (kb): 2.100
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.8, 0.3; PstI--3.0, 0.75, 0.54, 0.47, 0.1; HindIII--5.1.
EST01473 matches GenBank accession HUMMAP4 with 97.59% identity over the sequence range: 1-250.
References:

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

J C Venter, personal communication

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