| 产品名称： | pSLH8 |
|---|---|
| 商品货号： | TS160782 |
| Designations： | pSLH8 |
| Depositors： | DR Woods |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.0006999969482420 Vector: pSLH8 (plasmid) Promoters: Promoter lac Construction: pUC18, pKS3 Marker(s):ampR,indigo+ Construct size (kb): 4.000699996948242 Features: marker(s): ampR promoter: lac replicon: pMB1 |
| Applications： | contains sequence indole dioxygenase produces protein indole dioxygenase vector containing primer sites useful for sequencing vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/XbaI--2.7, 1.4; EcoRI--4.1; BamHI--4.1. The indigo pigment is intracellular and is produced on unsupplemented LB agar. Production is subject to catabolite repression by glucose, but is enhanced by tryptophan, indole, lactose, or IPTG. A high-copy-number cloning vector permitting visual detection of recombinants using insertional inactivation of the Rhodococcus gene for indigo production. Includes priming sites useful for sequencing. The M13 reverse sequencing primer and a 17mer primer (5-3) (CGAAGGCCGGTACCAGG) complementary to the 5 end of the pigment gene (nt 849-832) can be used for sequencing cloned DNA inserts. The order of the major features in this plasmid is: lacOP - EcoRI/MCS/SphI - indigo - lacZ remnant - ampR - pMB1 ori - lacI. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Hart S, Woods DR. Construction of an insertional-inactivation cloning vector for Escherichia coli using a Rhodococcus gene for indigo production. J. Gen. Microbiol. 138: 205-209, 1992. PubMed: 1556550 Hart S, et al. Structure of a Rhodococcus gene encoding pigment production in Escherichia coli. J. Gen. Microbiol. 136: 1357-1363, 1990. PubMed: 2230720 Hart S, et al. Identification of indigo-related pigments produced by Escherichia coli containing a cloned Rhodococcus gene. J. Gen. Microbiol. 138: 211-216, 1992. PubMed: 1372930 |
| Shipped： | freeze-dried |
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| 产品名称： | pSLH8 |
|---|---|
| 商品货号： | TS160782 |
| Designations： | pSLH8 |
| Depositors： | DR Woods |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.0006999969482420 Vector: pSLH8 (plasmid) Promoters: Promoter lac Construction: pUC18, pKS3 Marker(s):ampR,indigo+ Construct size (kb): 4.000699996948242 Features: marker(s): ampR promoter: lac replicon: pMB1 |
| Applications： | contains sequence indole dioxygenase produces protein indole dioxygenase vector containing primer sites useful for sequencing vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/XbaI--2.7, 1.4; EcoRI--4.1; BamHI--4.1. The indigo pigment is intracellular and is produced on unsupplemented LB agar. Production is subject to catabolite repression by glucose, but is enhanced by tryptophan, indole, lactose, or IPTG. A high-copy-number cloning vector permitting visual detection of recombinants using insertional inactivation of the Rhodococcus gene for indigo production. Includes priming sites useful for sequencing. The M13 reverse sequencing primer and a 17mer primer (5-3) (CGAAGGCCGGTACCAGG) complementary to the 5 end of the pigment gene (nt 849-832) can be used for sequencing cloned DNA inserts. The order of the major features in this plasmid is: lacOP - EcoRI/MCS/SphI - indigo - lacZ remnant - ampR - pMB1 ori - lacI. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Hart S, Woods DR. Construction of an insertional-inactivation cloning vector for Escherichia coli using a Rhodococcus gene for indigo production. J. Gen. Microbiol. 138: 205-209, 1992. PubMed: 1556550 Hart S, et al. Structure of a Rhodococcus gene encoding pigment production in Escherichia coli. J. Gen. Microbiol. 136: 1357-1363, 1990. PubMed: 2230720 Hart S, et al. Identification of indigo-related pigments produced by Escherichia coli containing a cloned Rhodococcus gene. J. Gen. Microbiol. 138: 211-216, 1992. PubMed: 1372930 |
| Shipped： | freeze-dried |
