| 产品名称： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
|---|---|
| 商品货号： | TS161296 |
| Deposited As： | Saccharomyces cerevisiae Hansen, teleomorph |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| Strain Designations： | YPH250 |
| Alternate State： | Candida robusta Diddens et Lodder |
| Application： | transformation host transformation host for expression of glyoxylase I, GloI transformation host for expression of Aspergillus saitoi carboxypeptidase gene |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Genotype： | MATa ura3-52 lys2-801 ade2-101 trp1-delta1 his3-delta200 leu2-delta1
Ref  Liu Y, et al. Preparation of high-activity whole cell biocatalysis by permeabilization of recombinant yeasts with alcohol. J. Biosci. Bioeng. 89: 554-558, 2000. |
| Preceptrol®： | no |
| Mating Type： | a |
| Ploidy： | haploid |
| Comments： | phytosphingosine-sensitive |
| Medium： | ATCC® Medium 1245: YEPD |
| Growth Conditions： | Temperature: 25.0°C |
| Name of Depositor： | P Heiter |
| Special Collection： | NCRR Contract |
| References： | Chiba Y, et al. Cloning and expression of the carboxypeptidase gene from Aspergillus saitoi and determination of the catalytic residues by site-directed mutagenesis. Biochem. J. 308: 405-409, 1995. PubMed: 7772020 Ostrander DB, Gorman JA. Characterization of the Candida albicans TRP1 gene and construction of a homozygous trp1 mutant by sequential co-transformation. Gene 148: 179-185, 1994. PubMed: 7958943 Nagiec MM, et al. The LCB2 gene of Saccharomyces and the related LCB1 gene encode subunits of serine palmitoyltransferase, the initial enzyme in sphingolipid synthesis. Proc. Natl. Acad. Sci. USA 91: 7899-7902, 1994. PubMed: 8058731 Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436 Liu Y, et al. Preparation of high-activity whole cell biocatalysis by permeabilization of recombinant yeasts with alcohol. J. Biosci. Bioeng. 89: 554-558, 2000. Liu Y, et al. Preparation of high-activity whole cell biocatalysis by permeabilization of recombinant yeasts with alcohol. J. Biosci. Bioeng. 89: 554-558, 2000. |
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| 产品名称： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
|---|---|
| 商品货号： | TS161296 |
| Deposited As： | Saccharomyces cerevisiae Hansen, teleomorph |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| Strain Designations： | YPH250 |
| Alternate State： | Candida robusta Diddens et Lodder |
| Application： | transformation host transformation host for expression of glyoxylase I, GloI transformation host for expression of Aspergillus saitoi carboxypeptidase gene |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Genotype： | MATa ura3-52 lys2-801 ade2-101 trp1-delta1 his3-delta200 leu2-delta1
RefLiu Y, et al. Preparation of high-activity whole cell biocatalysis by permeabilization of recombinant yeasts with alcohol. J. Biosci. Bioeng. 89: 554-558, 2000. |
| Preceptrol®： | no |
| Mating Type： | a |
| Ploidy： | haploid |
| Comments： | phytosphingosine-sensitive |
| Medium： | ATCC® Medium 1245: YEPD |
| Growth Conditions： | Temperature: 25.0°C |
| Name of Depositor： | P Heiter |
| Special Collection： | NCRR Contract |
| References： | Chiba Y, et al. Cloning and expression of the carboxypeptidase gene from Aspergillus saitoi and determination of the catalytic residues by site-directed mutagenesis. Biochem. J. 308: 405-409, 1995. PubMed: 7772020 Ostrander DB, Gorman JA. Characterization of the Candida albicans TRP1 gene and construction of a homozygous trp1 mutant by sequential co-transformation. Gene 148: 179-185, 1994. PubMed: 7958943 Nagiec MM, et al. The LCB2 gene of Saccharomyces and the related LCB1 gene encode subunits of serine palmitoyltransferase, the initial enzyme in sphingolipid synthesis. Proc. Natl. Acad. Sci. USA 91: 7899-7902, 1994. PubMed: 8058731 Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436 Liu Y, et al. Preparation of high-activity whole cell biocatalysis by permeabilization of recombinant yeasts with alcohol. J. Biosci. Bioeng. 89: 554-558, 2000. Liu Y, et al. Preparation of high-activity whole cell biocatalysis by permeabilization of recombinant yeasts with alcohol. J. Biosci. Bioeng. 89: 554-558, 2000. |
