Crithidia permixta-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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Crithidia permixta
Crithidia permixta
规格:
货期:
编号:TS162055
品牌:Testobio
产品名称： Crithidia permixta
商品货号： TS162055
Strain Designations： 128 SI
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Intestine of Mirini sp. (Insecta, Heteroptera, Miridae). Cosanga, Ecuador, 2005.
Product Format： frozen
Storage Conditions： Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain： no
Medium： ATCC® Medium 2831: SDM-79 Medium
ATCC® Medium 431: Trypanosome medium
Growth Conditions：
Temperature: 25°C
Culture System: Axenic
Cryopreservation：

Harvest and Preservation

  1. Harvest cells from a culture which is at or near peak density by centrifugation at 1,300 g for 5 min.
  2. Adjust concentration of cells to 2 x 107/mL in fresh medium.
  3. While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium (broth).xa0 The DMSO solution when first prepared will warm up due to chemical heat. The solution should be allowed to return to room temperature prior to use.
  4. Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/mL and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no more than 15 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the ampules in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0(The cooling rate in this apparatus is approximately -1°C/min.) xa0
  7. Store in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.xa0Do not agitate the ampule.xa0 Do not leave ampule in water bath after thawed.
  9. Immediately after thawing, do not leave in the water bath, aseptically transfer the contents of the ampule into a fresh flask containing 10 mLxa0ATCC Medium 2831.xa0
  10. Incubate at 25°C with the cap screwed on tightly.
  11. Maintain as described above. xa0
Name of Depositor： Dmitri Maslov
Year of Origin： 2005
References：

Yurchenko VY, et al. Morphological discordance of the new trypanosomatid species phylogenetically associated with the genus Crithidia. Protist 159: 99-114, 2008. PubMed: 17931968

Yurchenko, V, et al. An integrated morphological and molecular approach to a new species description in the Trypanosomatidae: the case of leptomonas podlipaevi n. sp., a parasite of Boisea rubrolineata (Hemiptera: Rhopalidae). J Eukaryot Microbiol. 53:103-111.

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Crithidia permixta

  • 货号: TS162055
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称： Crithidia permixta
商品货号： TS162055
Strain Designations： 128 SI
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Intestine of Mirini sp. (Insecta, Heteroptera, Miridae). Cosanga, Ecuador, 2005.
Product Format： frozen
Storage Conditions： Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain： no
Medium： ATCC® Medium 2831: SDM-79 Medium
ATCC® Medium 431: Trypanosome medium
Growth Conditions：
Temperature: 25°C
Culture System: Axenic
Cryopreservation：

Harvest and Preservation

  1. Harvest cells from a culture which is at or near peak density by centrifugation at 1,300 g for 5 min.
  2. Adjust concentration of cells to 2 x 107/mL in fresh medium.
  3. While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium (broth).xa0 The DMSO solution when first prepared will warm up due to chemical heat. The solution should be allowed to return to room temperature prior to use.
  4. Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/mL and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no more than 15 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the ampules in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0(The cooling rate in this apparatus is approximately -1°C/min.) xa0
  7. Store in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.xa0Do not agitate the ampule.xa0 Do not leave ampule in water bath after thawed.
  9. Immediately after thawing, do not leave in the water bath, aseptically transfer the contents of the ampule into a fresh flask containing 10 mLxa0ATCC Medium 2831.xa0
  10. Incubate at 25°C with the cap screwed on tightly.
  11. Maintain as described above. xa0
Name of Depositor： Dmitri Maslov
Year of Origin： 2005
References：

Yurchenko VY, et al. Morphological discordance of the new trypanosomatid species phylogenetically associated with the genus Crithidia. Protist 159: 99-114, 2008. PubMed: 17931968

Yurchenko, V, et al. An integrated morphological and molecular approach to a new species description in the Trypanosomatidae: the case of leptomonas podlipaevi n. sp., a parasite of Boisea rubrolineata (Hemiptera: Rhopalidae). J Eukaryot Microbiol. 53:103-111.

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