| 产品名称： | pCT29-8 |
|---|---|
| 商品货号： | TS162567 |
| Designations： | pCT29-8 |
| GenBank Number： | X12548 |
| Species： | Homo sapiens, human |
| Applications： | Studies of processing in this system suggest that the enzyme is synthesized and transported to lysosomes as a transmembrane protein with a short cytoplasmic tail. |
| Vector： | Construct size (kb): 5.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.111999988555908 Tissue: placenta Gene product: acid phosphatase 2, lysosomal ACP2 |
| Insert Size (kb)： | 2.112 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | PstI digestion also results in a 75 bp fragment not visible under standard conditions. Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.18; BamHI--4.55, 0.58; KpnI--uncut; HindIII--5.2; PstI--3.5, 1.08, 0.64. Insert encodes a 12 bp 5 untranslated region followed by a 1269 bp coding region. When the insert was cloned into pSVL and transfected into COS cells, acid phosphatase activity was expressed and 40% was membrane-associated. The EcoRI insert of pCT29-8 was inserted into pBEH and transfected into BHK cells. Studies of processing in this system suggest that the enzyme is synthesized and transported to lysosomes as a transmembrane protein with a short cytoplasmic tail. The insert detects a 2.3 kb mRNA in human liver and a 21 kb EcoRI fragment in human DNA. It detects a 5.0 kb EcoRI fragment in mouse DNA. |
| References： | Waheed A, et al. Human lysosomal acid phosphatase is transported as a transmembrane protein to lysosomes in tranfected baby hamster kidney cells. EMBO J. 7: 2351-2358, 1988. PubMed: 3056714 Pohlmann R, et al. Human lysosomal acid phosphatase: cloning, expression and chromosomal assignment. EMBO J. 7: 2343-2350, 1988. PubMed: 3191910 Regina Pohlmann, personal communication |
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| 产品名称： | pCT29-8 |
|---|---|
| 商品货号： | TS162567 |
| Designations： | pCT29-8 |
| GenBank Number： | X12548 |
| Species： | Homo sapiens, human |
| Applications： | Studies of processing in this system suggest that the enzyme is synthesized and transported to lysosomes as a transmembrane protein with a short cytoplasmic tail. |
| Vector： | Construct size (kb): 5.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.111999988555908 Tissue: placenta Gene product: acid phosphatase 2, lysosomal ACP2 |
| Insert Size (kb)： | 2.112 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | PstI digestion also results in a 75 bp fragment not visible under standard conditions. Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.18; BamHI--4.55, 0.58; KpnI--uncut; HindIII--5.2; PstI--3.5, 1.08, 0.64. Insert encodes a 12 bp 5 untranslated region followed by a 1269 bp coding region. When the insert was cloned into pSVL and transfected into COS cells, acid phosphatase activity was expressed and 40% was membrane-associated. The EcoRI insert of pCT29-8 was inserted into pBEH and transfected into BHK cells. Studies of processing in this system suggest that the enzyme is synthesized and transported to lysosomes as a transmembrane protein with a short cytoplasmic tail. The insert detects a 2.3 kb mRNA in human liver and a 21 kb EcoRI fragment in human DNA. It detects a 5.0 kb EcoRI fragment in mouse DNA. |
| References： | Waheed A, et al. Human lysosomal acid phosphatase is transported as a transmembrane protein to lysosomes in tranfected baby hamster kidney cells. EMBO J. 7: 2351-2358, 1988. PubMed: 3056714 Pohlmann R, et al. Human lysosomal acid phosphatase: cloning, expression and chromosomal assignment. EMBO J. 7: 2343-2350, 1988. PubMed: 3191910 Regina Pohlmann, personal communication |
