| 产品名称： | pVA517A THROUGH H |
|---|---|
| 商品货号： | TS162596 |
| Designations： | pVA517A THROUGH H |
| Depositors： | EM Lederberg |
| Other IDs： | Nucleotide (GenBank) : L04669 Saccharomyces cerevisiae (clone pSMD1-PRP38) D1 snRNP (SMD1) gene, |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 0E-16 Vector: pVA517A THROUGH H (plasmid) Construction: natural isolate Construct size (kb): 0.0 |
| Applications： | May be used as covalently-closed circular markers in agarose gel electrophoresis. |
| Comments： | Isolate plasmids from mid-log phase cells following the protocol in Biochemistry 9: 4428-4439, 1970 exactly. DNA preparations are best stored at -20C divided into small samples. natural isolate May be used as covalently-closed circular markers in agarose gel electrophoresis. This strain contains 8 plasmids: A--53.23 kb, B--7.16 kb, C--5.45 kb, D--5.04 kb, E--3.91 kb, F--3.02 kb, G--2.66 kb, H--2.02 kb. |
| Media： | ATCC® Medium 3: Nutrient agar or nutrient broth |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Macrina FL, et al. A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. Plasmid 1: 417-420, 1978. PubMed: 372973 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
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| 产品名称： | pVA517A THROUGH H |
|---|---|
| 商品货号： | TS162596 |
| Designations： | pVA517A THROUGH H |
| Depositors： | EM Lederberg |
| Other IDs： | Nucleotide (GenBank) : L04669 Saccharomyces cerevisiae (clone pSMD1-PRP38) D1 snRNP (SMD1) gene, |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 0E-16 Vector: pVA517A THROUGH H (plasmid) Construction: natural isolate Construct size (kb): 0.0 |
| Applications： | May be used as covalently-closed circular markers in agarose gel electrophoresis. |
| Comments： | Isolate plasmids from mid-log phase cells following the protocol in Biochemistry 9: 4428-4439, 1970 exactly. DNA preparations are best stored at -20C divided into small samples. natural isolate May be used as covalently-closed circular markers in agarose gel electrophoresis. This strain contains 8 plasmids: A--53.23 kb, B--7.16 kb, C--5.45 kb, D--5.04 kb, E--3.91 kb, F--3.02 kb, G--2.66 kb, H--2.02 kb. |
| Media： | ATCC® Medium 3: Nutrient agar or nutrient broth |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Macrina FL, et al. A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. Plasmid 1: 417-420, 1978. PubMed: 372973 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
