pBR322-C-A3 [pC-AC]
pBR322-C-A3 [pC-AC]
规格:
货期:
编号:TS162603
品牌:Testobio
| 产品名称： | pBR322-C-A3 pC-AC |
|---|---|
| 商品货号： | TS162603 |
| Designations： | pBR322-C-A3 pC-AC |
| GenBank Number： | M26602 |
| Species： | Homo sapiens, human |
| Depositors： | Univ. of Texas--M.D. Anderson Cancer Center, WM Mars, GF Saunders, Univ. of Texas--M.D. Anderson Cancer Center |
| Applications： | Can be used in Northern blots to detect mRNA abundant in chronic myelogenous leukemia but not in normal leukocytes. |
| Vector： | Construct size (kb): 4.630000114440918 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: PstI Vector end: PstI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: DEF1 Other gene symbols: MRS Genomic copy number: unique Gene name: defensin 1 Contains complete coding sequence?: U Chromosome: 8; Localization: 8 p23-p22 Tissue: chronic myelogenous leukemia, Philadelphia + Type of DNA: cDNA Insert end: Modification: GC tails Insert end: Modification: GC tails Insert size (kb): 0.33 Cross references: DNA Seq. Acc.: M26602 Insert lengths(kb): 0.3300000131130219 Tissue: chronic myelogenous leukemia, Philadelphia + Gene product: defensin 1 MRS |
| Insert Size (kb)： | 0.330 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following restriction sites (with nucleotide positions according to the published sequence): PstI--186 (close to 5 end of the insert), HindIII--246, SstI--403, RsaI--448, 493. Restriction digests of the clone give the following sizes (kb): PstI--4.6; EcoRI--4.6; PvuII--4.6; PstI/EcoRI--3.7, 0.9; PstI/PvuII--3.4, 1.4. The insert cannot be excised by PstI alone due to a deletion in the vector. Approximately 260 bp can be cut out using a PstI/RsaI double digest. Can be used in Northern blots to detect mRNA abundant in chronic myelogenous leukemia but not in normal leukocytes. |
| References： | Saunders GF, Mars WM. Probe for detection of specific human leukemias. US Patent 4,857,466 dated Aug 15 1989 Mars WM, et al. A myeloid-related sequence that localizes to human chromosome 8q21.1-22. Blood 71: 1713-1719, 1988. PubMed: 3370315 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pBR322-C-A3 pC-AC |
|---|---|
| 商品货号： | TS162603 |
| Designations： | pBR322-C-A3 pC-AC |
| GenBank Number： | M26602 |
| Species： | Homo sapiens, human |
| Depositors： | Univ. of Texas--M.D. Anderson Cancer Center, WM Mars, GF Saunders, Univ. of Texas--M.D. Anderson Cancer Center |
| Applications： | Can be used in Northern blots to detect mRNA abundant in chronic myelogenous leukemia but not in normal leukocytes. |
| Vector： | Construct size (kb): 4.630000114440918 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: PstI Vector end: PstI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: DEF1 Other gene symbols: MRS Genomic copy number: unique Gene name: defensin 1 Contains complete coding sequence?: U Chromosome: 8; Localization: 8 p23-p22 Tissue: chronic myelogenous leukemia, Philadelphia + Type of DNA: cDNA Insert end: Modification: GC tails Insert end: Modification: GC tails Insert size (kb): 0.33 Cross references: DNA Seq. Acc.: M26602 Insert lengths(kb): 0.3300000131130219 Tissue: chronic myelogenous leukemia, Philadelphia + Gene product: defensin 1 MRS |
| Insert Size (kb)： | 0.330 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following restriction sites (with nucleotide positions according to the published sequence): PstI--186 (close to 5 end of the insert), HindIII--246, SstI--403, RsaI--448, 493. Restriction digests of the clone give the following sizes (kb): PstI--4.6; EcoRI--4.6; PvuII--4.6; PstI/EcoRI--3.7, 0.9; PstI/PvuII--3.4, 1.4. The insert cannot be excised by PstI alone due to a deletion in the vector. Approximately 260 bp can be cut out using a PstI/RsaI double digest. Can be used in Northern blots to detect mRNA abundant in chronic myelogenous leukemia but not in normal leukocytes. |
| References： | Saunders GF, Mars WM. Probe for detection of specific human leukemias. US Patent 4,857,466 dated Aug 15 1989 Mars WM, et al. A myeloid-related sequence that localizes to human chromosome 8q21.1-22. Blood 71: 1713-1719, 1988. PubMed: 3370315 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
