| 产品名称： | Human rhinovirus 49 |
|---|---|
| 商品货号： | TS162685 |
| Classification： | Picornaviridae, Rhinovirus |
| Agent： | Human rhinovirus 49 |
| Strain： | 8213 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | This product is a mycoplasma free preparation derived from NIAID Research Reference Reagent V-116-002-021 (ATCC VR-1159). It replaces ATCC VR-1159 and ATCC VR-516, which will be discontinued when inventory is depleted. Strain 8213 is the prototype strain for human rhinovirus 49 and it has been fully sequenced (GenBank: DQ473496.1). |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Genome Sequenced Strain： | Yes |
| Comments： | This product is a mycoplasma free preparation derived from NIAID Research Reference Reagent V-116-002-021 (ATCC VR-1159). It replaces ATCC VR-1159 and ATCC VR-516, which will be discontinued when inventory is depleted. Strain 8213 is the prototype strain for human rhinovirus 49 and it has been fully sequenced (GenBank: DQ473496.1). |
| Effect on Host： | Typical rhinovirus CPE in cell culture, including rounding and sloughing of infected cells. |
| Growth Medium： | ATCC® Medium 30-2003: Eagles Minimum Essential Medium (EMEM) Fetal Bovine Serum |
| Recommended Host： | Production Host:H1-HeLa (ATCC CRL-1958) Production Host: H1-HeLa (ATCC® CRL-1958x99) Alternate Hosts:WI-38 (ATCC CCL-75 ), HeLa (ATCC CCL-2 ), KB (ATCC CCL-17 ) and other human cells. |
| Growth Conditions： | For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluence using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 33.0°C in a humidified, 5% CO2 atmosphere for 2-7 days with rocking, until CPE are well advanced in 100% of the culture. Harvest cells and culture fluids; cell debris can be removed by centrifugation if desired. Freeze preparation as rapidly as possible. |
| Name of Depositor： | NIAID, ATCC |
| Source： | Presumed from human throat washings; derived from ATCC VR-1159 following treatment to remove mycoplasma contamination. |
| References： | Fenters JD, et al. Propagation of Rhinovirus on WI-38 cell monolayers in rolling bottles. Appl. Microbiol. 15: 1460-1464, 1967. Kistler AL, et al. Genome-wide diversity and selective pressure in the human rhinovirus. Virol. J. 4: 40, 2007. PubMed: 17477878 |
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| 产品名称： | Human rhinovirus 49 |
|---|---|
| 商品货号： | TS162685 |
| Classification： | Picornaviridae, Rhinovirus |
| Agent： | Human rhinovirus 49 |
| Strain： | 8213 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | This product is a mycoplasma free preparation derived from NIAID Research Reference Reagent V-116-002-021 (ATCC VR-1159). It replaces ATCC VR-1159 and ATCC VR-516, which will be discontinued when inventory is depleted. Strain 8213 is the prototype strain for human rhinovirus 49 and it has been fully sequenced (GenBank: DQ473496.1). |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Genome Sequenced Strain： | Yes |
| Comments： | This product is a mycoplasma free preparation derived from NIAID Research Reference Reagent V-116-002-021 (ATCC VR-1159). It replaces ATCC VR-1159 and ATCC VR-516, which will be discontinued when inventory is depleted. Strain 8213 is the prototype strain for human rhinovirus 49 and it has been fully sequenced (GenBank: DQ473496.1). |
| Effect on Host： | Typical rhinovirus CPE in cell culture, including rounding and sloughing of infected cells. |
| Growth Medium： | ATCC® Medium 30-2003: Eagles Minimum Essential Medium (EMEM) Fetal Bovine Serum |
| Recommended Host： | Production Host:H1-HeLa (ATCC CRL-1958) Production Host: H1-HeLa (ATCC® CRL-1958x99) Alternate Hosts:WI-38 (ATCC CCL-75 ), HeLa (ATCC CCL-2 ), KB (ATCC CCL-17 ) and other human cells. |
| Growth Conditions： | For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluence using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 33.0°C in a humidified, 5% CO2 atmosphere for 2-7 days with rocking, until CPE are well advanced in 100% of the culture. Harvest cells and culture fluids; cell debris can be removed by centrifugation if desired. Freeze preparation as rapidly as possible. |
| Name of Depositor： | NIAID, ATCC |
| Source： | Presumed from human throat washings; derived from ATCC VR-1159 following treatment to remove mycoplasma contamination. |
| References： | Fenters JD, et al. Propagation of Rhinovirus on WI-38 cell monolayers in rolling bottles. Appl. Microbiol. 15: 1460-1464, 1967. Kistler AL, et al. Genome-wide diversity and selective pressure in the human rhinovirus. Virol. J. 4: 40, 2007. PubMed: 17477878 |
