| 产品名称： | pLES2 |
|---|---|
| 商品货号： | TS162878 |
| Designations： | pLES2 |
| Depositors： | DC Stein |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.0000000000000000 Vector: pLES2 (plasmid) Construction: pGC3, pUC9 lacZ Marker(s):ampR Construct size (kb): 6.0 Features: insert detection: lacZ marker(s): ampR replicon: pMB1, pSY1005 |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): SmaI--6.0; HindIII--2.4, 3.6; EcoRI--6.0; AvaI--4.0, 2.1. Contrary to the diagram in the reference, there are two AvaI sites on the vector. The beta-lactamase production phenotype functions in both Escherichia coli and N. gonorrhoeae. An Escherichia coli/Neisseria gonorrhoeae shuttle vector. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Vieira J, Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19: 259-268, 1982. PubMed: 6295879 Stein DC, et al. Construction and characterization of a new shuttle vector, pLES2, capable of functioning in Escherichia coli and Neisseria gonorrhoeae. Gene 25: 241-247, 1983. PubMed: 6319238 |
| Shipped： | freeze-dried |
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| 产品名称： | pLES2 |
|---|---|
| 商品货号： | TS162878 |
| Designations： | pLES2 |
| Depositors： | DC Stein |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.0000000000000000 Vector: pLES2 (plasmid) Construction: pGC3, pUC9 lacZ Marker(s):ampR Construct size (kb): 6.0 Features: insert detection: lacZ marker(s): ampR replicon: pMB1, pSY1005 |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): SmaI--6.0; HindIII--2.4, 3.6; EcoRI--6.0; AvaI--4.0, 2.1. Contrary to the diagram in the reference, there are two AvaI sites on the vector. The beta-lactamase production phenotype functions in both Escherichia coli and N. gonorrhoeae. An Escherichia coli/Neisseria gonorrhoeae shuttle vector. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Vieira J, Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19: 259-268, 1982. PubMed: 6295879 Stein DC, et al. Construction and characterization of a new shuttle vector, pLES2, capable of functioning in Escherichia coli and Neisseria gonorrhoeae. Gene 25: 241-247, 1983. PubMed: 6319238 |
| Shipped： | freeze-dried |
