pJJ281 [pJJ248]-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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pJJ281 [pJJ248]
pJJ281 [pJJ248]
规格:
货期:
编号:TS162936
品牌:Testobio
产品名称: pJJ281 pJJ248
商品货号: TS162936
Designations: pJJ281 pJJ248
Species: Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors: L Prakash
Vector:
Construct size (kb): 3.700000047683716
Insert:
DNA: genomic
Insert lengths(kb): 0.9359999895095825
Gene product: phosphoribosylanthranilate isomerase TRP1
Insert Size (kb): 0.936
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): HindIII/BamHI--2.7, 0.7, 0.4; PvuII--2.4, 1.3; EcoRI--3.7.
A plasmid providing the gene for a yeast selectable marker in the MCS of pUC18, allowing more flexibility in designing constructions.
Constructed by filling in the ends of both the vector and the insert fragment.
TRP1 is in the same orientation as lacZ.
The insert was subcloned from YRp17.
Classification: Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References:

Jones JS, Prakash L. Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers. Yeast 6: 363-366, 1990. PubMed: 2220072

Louise Prakash, personal communication

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pJJ281 [pJJ248]

  • 货号: TS162936
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  • 品牌 : TESTOBIO
产品名称: pJJ281 pJJ248
商品货号: TS162936
Designations: pJJ281 pJJ248
Species: Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors: L Prakash
Vector:
Construct size (kb): 3.700000047683716
Insert:
DNA: genomic
Insert lengths(kb): 0.9359999895095825
Gene product: phosphoribosylanthranilate isomerase TRP1
Insert Size (kb): 0.936
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): HindIII/BamHI--2.7, 0.7, 0.4; PvuII--2.4, 1.3; EcoRI--3.7.
A plasmid providing the gene for a yeast selectable marker in the MCS of pUC18, allowing more flexibility in designing constructions.
Constructed by filling in the ends of both the vector and the insert fragment.
TRP1 is in the same orientation as lacZ.
The insert was subcloned from YRp17.
Classification: Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References:

Jones JS, Prakash L. Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers. Yeast 6: 363-366, 1990. PubMed: 2220072

Louise Prakash, personal communication

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