pKi-67/1-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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pKi-67/1
pKi-67/1
规格:
货期:
编号:TS163006
品牌:Testobio
产品名称: pKi-67/1
商品货号: TS163006
Designations: pKi-67/1
GenBank Number:

X65550

Species: Homo sapiens, human
Depositors: J Gerdes
Vector:
Construct size (kb): 6.800000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR325
Intact vector size: 5.996
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: AatII AsuII AvaI ClaI HgiEII HindIII Tth111I BamHI EcoRI
EcoRV (MstI-Cm?) NcoI PstI PvuI SalI SphI XmaIII XmnI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmLR, tetR
replicon: pMB1
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MKI67
Gene name: antigen identified by monoclonal antibody Ki-67
Contains complete coding sequence?: N
Chromosome: 10; Localization: 10 q25-qter
Tissue: IM-9 cell line
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.092
Cross references: DNA Seq. Acc.: X65550
Insert lengths(kb): 1.092000007629395
Tissue: IM-9 cell line
Gene product: antigen identified by monoclonal antibody Ki-67( Proliferation-related Ki-67 antigen, Ki-67 antigen) MKI67
Insert Size (kb): 1.092
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--6.0, 1.1; HindIII--7.0; PstI--5.0, 2.1; SacI--7.1; XbaI--7.1.
The insert contains the following restriction sites (approximate kb from the 5 end): PstI--1.00; SacI--0.21, 0.57, 0.73, 0.94; XbaI--0.18.
It includes three of sixteen Ki-67 repeats.
Detects splicing isoforms of 8.7 and 9.8 kb.
References:

Fonatsch C, et al. Assignment of the human Ki-67 gene (MKI67) to 10q25-qter. Genomics 11: 476-477, 1991. PubMed: 1769665

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pKi-67/1

  • 货号: TS163006
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: pKi-67/1
商品货号: TS163006
Designations: pKi-67/1
GenBank Number:

X65550

Species: Homo sapiens, human
Depositors: J Gerdes
Vector:
Construct size (kb): 6.800000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR325
Intact vector size: 5.996
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: AatII AsuII AvaI ClaI HgiEII HindIII Tth111I BamHI EcoRI
EcoRV (MstI-Cm?) NcoI PstI PvuI SalI SphI XmaIII XmnI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmLR, tetR
replicon: pMB1
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MKI67
Gene name: antigen identified by monoclonal antibody Ki-67
Contains complete coding sequence?: N
Chromosome: 10; Localization: 10 q25-qter
Tissue: IM-9 cell line
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.092
Cross references: DNA Seq. Acc.: X65550
Insert lengths(kb): 1.092000007629395
Tissue: IM-9 cell line
Gene product: antigen identified by monoclonal antibody Ki-67( Proliferation-related Ki-67 antigen, Ki-67 antigen) MKI67
Insert Size (kb): 1.092
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--6.0, 1.1; HindIII--7.0; PstI--5.0, 2.1; SacI--7.1; XbaI--7.1.
The insert contains the following restriction sites (approximate kb from the 5 end): PstI--1.00; SacI--0.21, 0.57, 0.73, 0.94; XbaI--0.18.
It includes three of sixteen Ki-67 repeats.
Detects splicing isoforms of 8.7 and 9.8 kb.
References:

Fonatsch C, et al. Assignment of the human Ki-67 gene (MKI67) to 10q25-qter. Genomics 11: 476-477, 1991. PubMed: 1769665

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