Monosiga brevicollis Ruinen-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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Monosiga brevicollis Ruinen
Monosiga brevicollis Ruinen
规格:
货期:
编号:TS163609
品牌:Testobio
产品名称： Monosiga brevicollis Ruinen
商品货号： TS163609
Strain Designations： MX1
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： ATCC® 50154™
Product Format： frozen
Storage Conditions： Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain： no
Genome Sequenced Strain：

Yes

Comments：
Genome strain developed from ATCC 50154
Contains Flavobacterium sp. as single bacterial food source
Genome sequencing strain (DOE)
Medium： ATCC® Medium 1525: Seawater 802 medium
Growth Conditions：
Temperature: 25°C
Cryopreservation： Reagents
Cryoprotective Solution
DMSO:xa02.0 mL
Fresh growth medium w/o bacteria:xa08.0 mL

Harvesting and Preservation
  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.
  2. Harvest cells from a culture that is at or near peak density by filtration and centrifugation at 800 x g for 5 min.
  3. Adjust the concentration of cells at least 2 x 106/mL in fresh medium.
  4. Mix the cell preparation and the cryoprotective solution in equal portions.
  5. Dispense in 0.5 mL aliquots into 1.0 mL toxa02.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.)xa0xa0
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place the vial in a 35°C water bath.xa0 Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.xa0xa0 Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate into a T-25 tissue culture flask containing 10 mL uninoculated ATCC medium 1525.
  9. Incubate at 25°C with the cap screwed on tightly.
  10. Once the culture is established, vigorously agitate the flask and aseptically transfer 0.5 mL to 10.0 mL of uninoculated ATCC medium 1525.
  11. Follow the protocol for maintenance of culture.
Name of Depositor： Nicole King
References：

Abedin M and King N. The premetazoan ancestry of cadherins. Science 319:946-948, 2008. PubMed:18276888

King N, et al. The genome of the choanoflagellate Monosiga brevicollis and the origin of metazoans. Nature 451:783-788, 2008. PubMed:18273011

Cross References：

Nucleotide (GenBank) : ABFJ01000000 Monosiga brevicollis MX1, whole genome shotgun sequencing project.

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Monosiga brevicollis Ruinen

  • 货号: TS163609
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： Monosiga brevicollis Ruinen
商品货号： TS163609
Strain Designations： MX1
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： ATCC® 50154™
Product Format： frozen
Storage Conditions： Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain： no
Genome Sequenced Strain：

Yes

Comments：
Genome strain developed from ATCC 50154
Contains Flavobacterium sp. as single bacterial food source
Genome sequencing strain (DOE)
Medium： ATCC® Medium 1525: Seawater 802 medium
Growth Conditions：
Temperature: 25°C
Cryopreservation： Reagents
Cryoprotective Solution
DMSO:xa02.0 mL
Fresh growth medium w/o bacteria:xa08.0 mL

Harvesting and Preservation
  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.
  2. Harvest cells from a culture that is at or near peak density by filtration and centrifugation at 800 x g for 5 min.
  3. Adjust the concentration of cells at least 2 x 106/mL in fresh medium.
  4. Mix the cell preparation and the cryoprotective solution in equal portions.
  5. Dispense in 0.5 mL aliquots into 1.0 mL toxa02.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.)xa0xa0
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place the vial in a 35°C water bath.xa0 Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.xa0xa0 Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate into a T-25 tissue culture flask containing 10 mL uninoculated ATCC medium 1525.
  9. Incubate at 25°C with the cap screwed on tightly.
  10. Once the culture is established, vigorously agitate the flask and aseptically transfer 0.5 mL to 10.0 mL of uninoculated ATCC medium 1525.
  11. Follow the protocol for maintenance of culture.
Name of Depositor： Nicole King
References：

Abedin M and King N. The premetazoan ancestry of cadherins. Science 319:946-948, 2008. PubMed:18276888

King N, et al. The genome of the choanoflagellate Monosiga brevicollis and the origin of metazoans. Nature 451:783-788, 2008. PubMed:18273011

Cross References：

Nucleotide (GenBank) : ABFJ01000000 Monosiga brevicollis MX1, whole genome shotgun sequencing project.

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