| 产品名称： | pGB29 |
|---|---|
| 商品货号： | TS165870 |
| Designations： | pGB29 |
| GenBank Number： | J05411 |
| Species： | Elizabethkingia miricola |
| Depositors： | Genzyme Corp., G Barsomian, Genzyme Corp. |
| Applications： | produces protein peptide-N(4)-(N-acetyl-beta-D-glucosaminyl) asparagine amidase F |
| Vector： | Construct size (kb): 6.200 |
| Insert： | DNA: genomic Insert lengths(kb): 3.30 Gene product: peptide-N(4)-(N-acetyl-beta-D-glucosaminyl) asparagine amidase F ngl Target Gene: peptide-N(4)-(N-acetyl-beta-D-glucosaminyl) asparagine amidase F |
| Insert Size (kb)： | 3.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the phagemid. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--uncut; Sau3AI--1.0, 0.7, 0.6, + smaller; BglII--3.5, 3.2. The sequenced portion of the insert contains the following restriction sites (approximate kb from the 5 end): EcoRV--1.33; HindIII--0.80; NdeI--0.85; PvuII--0.93; EcoRI--1.6. Additional EcoRI sites are known to occur beyond the site indicated. Contains the complete coding sequence (nt 139-1203). A subclone deleting EcoRI fragments downstream of the gene was used for sequencing and expression studies. Proteins of 35, 37 and 38 kDa are expressed. Expression is independent of orientation in the vector, suggesting the presence of an endogenous promoter that functions in E. coli. The vector was constructed from pBluescript SK+ by converting ApaI and SacI sites into BglII sites. The insert in this clone was isolated from ATCC 33958, Elizabethkingia miricola, formerly Flavobacterium meningosepticum. |
| References： | Barsomian GD, et al. Cloning and expression of peptide-N(4)-(N-acetyl-beta-D-glucosaminyl) asparagine amidase F in Escherichia coli. J. Biol. Chem. 265: 6967-6972, 1990. PubMed: 2182635 Barsomian GD, et al. Endo F-free PNGase. US Patent 5,238,821 dated Aug 24 1993 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pGB29 |
|---|---|
| 商品货号： | TS165870 |
| Designations： | pGB29 |
| GenBank Number： | J05411 |
| Species： | Elizabethkingia miricola |
| Depositors： | Genzyme Corp., G Barsomian, Genzyme Corp. |
| Applications： | produces protein peptide-N(4)-(N-acetyl-beta-D-glucosaminyl) asparagine amidase F |
| Vector： | Construct size (kb): 6.200 |
| Insert： | DNA: genomic Insert lengths(kb): 3.30 Gene product: peptide-N(4)-(N-acetyl-beta-D-glucosaminyl) asparagine amidase F ngl Target Gene: peptide-N(4)-(N-acetyl-beta-D-glucosaminyl) asparagine amidase F |
| Insert Size (kb)： | 3.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the phagemid. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--uncut; Sau3AI--1.0, 0.7, 0.6, + smaller; BglII--3.5, 3.2. The sequenced portion of the insert contains the following restriction sites (approximate kb from the 5 end): EcoRV--1.33; HindIII--0.80; NdeI--0.85; PvuII--0.93; EcoRI--1.6. Additional EcoRI sites are known to occur beyond the site indicated. Contains the complete coding sequence (nt 139-1203). A subclone deleting EcoRI fragments downstream of the gene was used for sequencing and expression studies. Proteins of 35, 37 and 38 kDa are expressed. Expression is independent of orientation in the vector, suggesting the presence of an endogenous promoter that functions in E. coli. The vector was constructed from pBluescript SK+ by converting ApaI and SacI sites into BglII sites. The insert in this clone was isolated from ATCC 33958, Elizabethkingia miricola, formerly Flavobacterium meningosepticum. |
| References： | Barsomian GD, et al. Cloning and expression of peptide-N(4)-(N-acetyl-beta-D-glucosaminyl) asparagine amidase F in Escherichia coli. J. Biol. Chem. 265: 6967-6972, 1990. PubMed: 2182635 Barsomian GD, et al. Endo F-free PNGase. US Patent 5,238,821 dated Aug 24 1993 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
