Plasmodium berghei Vincke and Lips-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

你好，请登录   免费注册    |    收藏本站
联系电话: 0574-87917803
你好,请登录   免费注册 | 收藏
联系电话: 0574-87917803
热门搜索: 金黄色葡萄球菌 铜绿假单胞菌 红菇 香菇
Plasmodium berghei Vincke and Lips
Plasmodium berghei Vincke and Lips
规格:
货期:
编号:TS166114
品牌:Testobio
产品名称： Plasmodium berghei Vincke and Lips
商品货号： TS166114
Strain Designations： NK65D-CLBB13
Application：
Vector borne research
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：
derived from M. Yoeli strain NK65 by mosquito passage, Univ. Illinois, Urbana, pre-1978
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic： Axenic
Type Strain： no
Cryopreservation：

Only young cells (rings) can be frozen in glycerolyte medium* because their membranes are more robust.

  1. To harvest parasites, inject host with ketamine (0.1-0.2 ml).
  2. Open chest cavity to expose heart and exsanguinate via cardiac puncture using Yaegers anticoagulant** (see below), 1 volume anticoagulant to 4 volumes blood.
  3. Centrifuge blood for 5 mins. at 1800 rpm in 50 ml centrifuge tube.
  4. Aspirate supernatant using sterile Pasteur pipet.
  5. Resuspend pellet gently in remaining supernatant.
  6. Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet dropwise via a syringe as follows:
    1. Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.
    2. Add the remaining 4 volumes of glycerolyte and gently agitate.
  7. Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1°C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.).
  8. Plunge vials into liquid nitrogen (-196°C) the next day and store in liquid nitrogen or liquid nitrogen vapor.
Name of Depositor： NE Alger
Year of Origin： 1978
References：

Branton MBStudies of clonal populations of NK65 Plasmodium berghei Ph.D. thesis, Univ. Illinois, 1978

首页 > 产品中心 > 微生物培养 > 菌株 > null > Plasmodium berghei Vincke and Lips

产品中心 Product Center

联系我们CONTACT US

  • 0574-87917803
    24小时服务热线
  • testobio@163.com
    销售邮箱
  • 浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
    地址

联系方式

  • 章丽敏
    17757489010
  • 朱静
    18067106081
  • 李晴
    18067408036
  • 李晶晶
    13375187189
  • 周雪
    13372109301

Plasmodium berghei Vincke and Lips

  • 货号: TS166114
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： Plasmodium berghei Vincke and Lips
商品货号： TS166114
Strain Designations： NK65D-CLBB13
Application：
Vector borne research
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：
derived from M. Yoeli strain NK65 by mosquito passage, Univ. Illinois, Urbana, pre-1978
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic： Axenic
Type Strain： no
Cryopreservation：

Only young cells (rings) can be frozen in glycerolyte medium* because their membranes are more robust.

  1. To harvest parasites, inject host with ketamine (0.1-0.2 ml).
  2. Open chest cavity to expose heart and exsanguinate via cardiac puncture using Yaegers anticoagulant** (see below), 1 volume anticoagulant to 4 volumes blood.
  3. Centrifuge blood for 5 mins. at 1800 rpm in 50 ml centrifuge tube.
  4. Aspirate supernatant using sterile Pasteur pipet.
  5. Resuspend pellet gently in remaining supernatant.
  6. Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet dropwise via a syringe as follows:
    1. Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.
    2. Add the remaining 4 volumes of glycerolyte and gently agitate.
  7. Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1°C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.).
  8. Plunge vials into liquid nitrogen (-196°C) the next day and store in liquid nitrogen or liquid nitrogen vapor.
Name of Depositor： NE Alger
Year of Origin： 1978
References：

Branton MBStudies of clonal populations of NK65 Plasmodium berghei Ph.D. thesis, Univ. Illinois, 1978

扫码关注小红书账号
关注我们
联系电话：0574-87917803
章丽敏：17757489010
朱静：18067106081
李晴：18067408036
李晶晶：13375187189
周雪：13372109301
邮箱：testobio@163.com
地址：浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
在线留言
合作单位: