pPW71B [PW71B]
pPW71B [PW71B]
规格:
货期:
编号:TS166162
品牌:Testobio
| 产品名称： | pPW71B PW71B |
|---|---|
| 商品货号： | TS166162 |
| Designations： | pPW71B PW71B |
| Species： | Homo sapiens, human |
| Applications： | contains sequence useful for DNA diagnostics DNA Segment, single copy probes |
| Vector： | Construct size (kb): 3.049999952316284 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC19 Intact vector size: 2.686 Type of vector: plasmid Vector end: SmaI Vector end: SmaI Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: EcoRI...HindIII, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: X02514 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: D15S63 Gene name: DNA Segment, single copy probes Contains complete coding sequence?: N Chromosome: 15; Localization: 15 q11-q12 Type of DNA: genomic Insert end: HaeIII Insert end: HaeIII Insert size (kb): 0.365 Cross references: Insert lengths(kb): 0.3650000095367432 Gene product: DNA Segment, single copy probes D15S63 Target Gene: DNA Segment, single copy probes |
| Insert Size (kb)： | 0.365 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.8, 0.44; PstI--3.2, PvuII--2.5, 0.76; SacI--3.2; XbaI--3.2. Insert detects parent-of-origin specific DNA methylation patterns in the Prader-Willi syndrome/Angelman syndrome chromosomal region (15 q11-q13). An imprinted HpaII and CfoI site in this region results in the maternal and paternal pattern. Patients with Prader-Willi syndrome typically lack the paternal bands. Lack of the maternal bands is indicative of Angelman syndrome. Because of the small probe size, low-stringent post-hybridization washes should be applied. |
| References： | Dittrich B, et al. Characterization of a methylation imprint in the Prader-Willi syndrome chromosome region. Hum. Mol. Genet. 2: 1995-1999, 1993. PubMed: 8111366 |
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13372109301
| 产品名称： | pPW71B PW71B |
|---|---|
| 商品货号： | TS166162 |
| Designations： | pPW71B PW71B |
| Species： | Homo sapiens, human |
| Applications： | contains sequence useful for DNA diagnostics DNA Segment, single copy probes |
| Vector： | Construct size (kb): 3.049999952316284 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC19 Intact vector size: 2.686 Type of vector: plasmid Vector end: SmaI Vector end: SmaI Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: EcoRI...HindIII, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: X02514 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: D15S63 Gene name: DNA Segment, single copy probes Contains complete coding sequence?: N Chromosome: 15; Localization: 15 q11-q12 Type of DNA: genomic Insert end: HaeIII Insert end: HaeIII Insert size (kb): 0.365 Cross references: Insert lengths(kb): 0.3650000095367432 Gene product: DNA Segment, single copy probes D15S63 Target Gene: DNA Segment, single copy probes |
| Insert Size (kb)： | 0.365 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.8, 0.44; PstI--3.2, PvuII--2.5, 0.76; SacI--3.2; XbaI--3.2. Insert detects parent-of-origin specific DNA methylation patterns in the Prader-Willi syndrome/Angelman syndrome chromosomal region (15 q11-q13). An imprinted HpaII and CfoI site in this region results in the maternal and paternal pattern. Patients with Prader-Willi syndrome typically lack the paternal bands. Lack of the maternal bands is indicative of Angelman syndrome. Because of the small probe size, low-stringent post-hybridization washes should be applied. |
| References： | Dittrich B, et al. Characterization of a methylation imprint in the Prader-Willi syndrome chromosome region. Hum. Mol. Genet. 2: 1995-1999, 1993. PubMed: 8111366 |
