| 产品名称： | Rotavirus A |
|---|---|
| 商品货号： | TS166985 |
| Classification： | Reoviridae, Rotavirus, Rotavirus A |
| Deposited As： | Human rotavirus, strain Hu/Australia/10-25-10/77L |
| Agent： | Rotavirus A |
| Strain： | Hu/Australia/102510/77/L |
| Applications： | Enteric Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | xa0-70°C or colder |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Comments： | This item is a Group A rotavirus with serotype: G3P2A6, showing typical rotavirus morphology by electron microscopy. It has a diameter of 65-75 nm for the double-shelled form and 55-65 nm for the single-shelled form. This virus appears to be attenuated in tissue culture. This item requires a freeze-thaw and reactivation of virus using trypsin to process intermediate passages. |
| Effect on Host： | CPE; cell clumping, refractile rounding and sloughing |
| Recommended Host： | MA-104 Clone 1 (ATCC CRL-2378.1) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 98-100% confluent. Calculate volume of virus seed required to inoculate with an optimal MOI (e.g. 0.2-0.4). Activate virus by incubating in 10 µg/mL Trypsin 1:250 within a 37°C water bath for 30 minutes. If needed, combine activated virus with enough virus growth medium to inoculate with a small amount of inoculum (e.g. 1 mL per 25 cm2). Wash cell monolayer twice with DPBS or EMEM. Adsorb activated virus for 1-2 hours at 37°C, in a humidified 5% CO2 environment with slow continuous rocking. End adsorption by removing the inoculum and then adding virus growth medium. Incubation: 12 days |
| Name of Depositor： | G Barnes |
| U.S. Patent： | |
| U.S. Patent Number： | |
| Source： | Stool sample of a human neonate in a Melbourne maternity hospital, 1977 |
| Year of Origin： | 1977 |
| References： | Albert MJ, et al. Live attenuated human rotavirus vaccine. US Pat. 4,624,850 dated Nov 25, 1986. Barnes GL, et al. Early phase II trial of human rotavirus vaccine candidate RV3. Vaccine. (20): 2950-2956, 2002. PubMed: 12126907 Dyall-Smith ML, et al. Sequence homology between human and animal rotavirus serotype-specific glycoproteins. Nucleic Acids Res. 12(9):3973-3982, 1984 |
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| 产品名称： | Rotavirus A |
|---|---|
| 商品货号： | TS166985 |
| Classification： | Reoviridae, Rotavirus, Rotavirus A |
| Deposited As： | Human rotavirus, strain Hu/Australia/10-25-10/77L |
| Agent： | Rotavirus A |
| Strain： | Hu/Australia/102510/77/L |
| Applications： | Enteric Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | xa0-70°C or colder |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Comments： | This item is a Group A rotavirus with serotype: G3P2A6, showing typical rotavirus morphology by electron microscopy. It has a diameter of 65-75 nm for the double-shelled form and 55-65 nm for the single-shelled form. This virus appears to be attenuated in tissue culture. This item requires a freeze-thaw and reactivation of virus using trypsin to process intermediate passages. |
| Effect on Host： | CPE; cell clumping, refractile rounding and sloughing |
| Recommended Host： | MA-104 Clone 1 (ATCC CRL-2378.1) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 98-100% confluent. Calculate volume of virus seed required to inoculate with an optimal MOI (e.g. 0.2-0.4). Activate virus by incubating in 10 µg/mL Trypsin 1:250 within a 37°C water bath for 30 minutes. If needed, combine activated virus with enough virus growth medium to inoculate with a small amount of inoculum (e.g. 1 mL per 25 cm2). Wash cell monolayer twice with DPBS or EMEM. Adsorb activated virus for 1-2 hours at 37°C, in a humidified 5% CO2 environment with slow continuous rocking. End adsorption by removing the inoculum and then adding virus growth medium. Incubation: 12 days |
| Name of Depositor： | G Barnes |
| U.S. Patent： | |
| U.S. Patent Number： | |
| Source： | Stool sample of a human neonate in a Melbourne maternity hospital, 1977 |
| Year of Origin： | 1977 |
| References： | Albert MJ, et al. Live attenuated human rotavirus vaccine. US Pat. 4,624,850 dated Nov 25, 1986. Barnes GL, et al. Early phase II trial of human rotavirus vaccine candidate RV3. Vaccine. (20): 2950-2956, 2002. PubMed: 12126907 Dyall-Smith ML, et al. Sequence homology between human and animal rotavirus serotype-specific glycoproteins. Nucleic Acids Res. 12(9):3973-3982, 1984 |
