p415 GAL1
p415 GAL1
规格:
货期:
编号:TS167252
品牌:Testobio
| 产品名称： | p415 GAL1 |
|---|---|
| 商品货号： | TS167252 |
| Designations： | p415 GAL1 |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 6.7069997787475590 DESCRIPTION OF VECTOR: Intact vector size: 6.707 Type of vector: plasmid Cloning sites: XbaI SpeI BamHI SmaI PstI HindIII SalI XhoI Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI Construction: pRS415, GAL1 promoter Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): LEU2, ->, 200-2446 terminator: CYC1, ->, 3128-3388 MCS: XhoI...XbaI, ->, 3388-3451 promoter for expression: GAL1, marker(s): ampR, centromere: CEN6/ARSH4, ->, 6193-6707 Vector: p415 GAL1 (plasmid) Promoters: Promoter for expression GAL1 Construction: pRS415, GAL1 promoter Marker(s):LEU2,ampR Construct size (kb): 6.706999778747559 Features: marker(s): LEU2 marker(s): ampR promoter for expression: GAL1 MCS: XhoI...XbaI terminator: CYC1 centromere: CEN6/ARSH4 |
| Applications： | YX-type (expression) shuttle vector vector with low copy number |
| Comments： | Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose. Low copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736 |
| Related Products： | component of:ATCC 87670 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |
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| 产品名称： | p415 GAL1 |
|---|---|
| 商品货号： | TS167252 |
| Designations： | p415 GAL1 |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 6.7069997787475590 DESCRIPTION OF VECTOR: Intact vector size: 6.707 Type of vector: plasmid Cloning sites: XbaI SpeI BamHI SmaI PstI HindIII SalI XhoI Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI Construction: pRS415, GAL1 promoter Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): LEU2, ->, 200-2446 terminator: CYC1, ->, 3128-3388 MCS: XhoI...XbaI, ->, 3388-3451 promoter for expression: GAL1, marker(s): ampR, centromere: CEN6/ARSH4, ->, 6193-6707 Vector: p415 GAL1 (plasmid) Promoters: Promoter for expression GAL1 Construction: pRS415, GAL1 promoter Marker(s):LEU2,ampR Construct size (kb): 6.706999778747559 Features: marker(s): LEU2 marker(s): ampR promoter for expression: GAL1 MCS: XhoI...XbaI terminator: CYC1 centromere: CEN6/ARSH4 |
| Applications： | YX-type (expression) shuttle vector vector with low copy number |
| Comments： | Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose. Low copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736 |
| Related Products： | component of:ATCC 87670 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |
