| 产品名称： | pB3HE1 |
|---|---|
| 商品货号： | TS167801 |
| Designations： | pB3HE1 |
| Species： | Brome mosaic virus, Brome mosaic virus |
| Depositors： | P Ahlquist |
| Applications： | Contains a noncoding sequence to serve as a probe for brome mosaic virus. The insert corresponds to the 3 200 bases of BMV RNA3. Because this region is 97-99% conserved among the BMV RNAs, the SP6 transcript of HindIII-linearized pB3HE1 hybridizes to all four BMV RNAs. |
| Vector： | Construct size (kb): 3.099999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.2000000029802322 Gene product: noncoding sequence |
| Insert Size (kb)： | 0.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the plasmid. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.9, 0.2; EcoRI--2.9, 0.2. EcoRI/HindIII digest on acrylamide gel gives the same sizes as above. Contains a noncoding sequence to serve as a probe for brome mosaic virus. The insert corresponds to the 3 200 bases of BMV RNA3. Because this region is 97-99% conserved among the BMV RNAs, the SP6 transcript of HindIII-linearized pB3HE1 hybridizes to all four BMV RNAs. Derived from the 3 200 bp HindIII/EcoRI fragment of pB3PM1. |
| Classification： | Bromoviridae, Bromovirus |
| References： | French R, Ahlquist P. Intercistronic as well as terminal sequences are required for efficient amplification of brome mosaic virus RNA3. J. Virol. 61: 1457-1465, 1987. PubMed: 3573144 Ahlquist P, Janda M. cDNA cloning and in vitro transcription of the complete brome mosaic virus genome. Mol. Cell. Biol. 4: 2876-2882, 1984. PubMed: 6549346 Ahlquist P, et al. Near identity of 3 RNA secondary structure in bromoviruses and cucumber mosaic virus. Cell 23: 183-189, 1981. PubMed: 7214524 |
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| 产品名称： | pB3HE1 |
|---|---|
| 商品货号： | TS167801 |
| Designations： | pB3HE1 |
| Species： | Brome mosaic virus, Brome mosaic virus |
| Depositors： | P Ahlquist |
| Applications： | Contains a noncoding sequence to serve as a probe for brome mosaic virus. The insert corresponds to the 3 200 bases of BMV RNA3. Because this region is 97-99% conserved among the BMV RNAs, the SP6 transcript of HindIII-linearized pB3HE1 hybridizes to all four BMV RNAs. |
| Vector： | Construct size (kb): 3.099999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.2000000029802322 Gene product: noncoding sequence |
| Insert Size (kb)： | 0.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the plasmid. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.9, 0.2; EcoRI--2.9, 0.2. EcoRI/HindIII digest on acrylamide gel gives the same sizes as above. Contains a noncoding sequence to serve as a probe for brome mosaic virus. The insert corresponds to the 3 200 bases of BMV RNA3. Because this region is 97-99% conserved among the BMV RNAs, the SP6 transcript of HindIII-linearized pB3HE1 hybridizes to all four BMV RNAs. Derived from the 3 200 bp HindIII/EcoRI fragment of pB3PM1. |
| Classification： | Bromoviridae, Bromovirus |
| References： | French R, Ahlquist P. Intercistronic as well as terminal sequences are required for efficient amplification of brome mosaic virus RNA3. J. Virol. 61: 1457-1465, 1987. PubMed: 3573144 Ahlquist P, Janda M. cDNA cloning and in vitro transcription of the complete brome mosaic virus genome. Mol. Cell. Biol. 4: 2876-2882, 1984. PubMed: 6549346 Ahlquist P, et al. Near identity of 3 RNA secondary structure in bromoviruses and cucumber mosaic virus. Cell 23: 183-189, 1981. PubMed: 7214524 |
