| 产品名称： | pHG30 |
|---|---|
| 商品货号： | TS167957 |
| Designations： | pHG30 |
| GenBank Number： | L36537 |
| Species： | Homo sapiens, human |
| Depositors： | H Schachter |
| Vector： | Construct size (kb): 5.960000038146973 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript II KS+ Intact vector size: 2.961 Type of vector: phagemid Vector end: XhoI Vector end: XhoI Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR replicon: pMB1, f1 Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: MGAT2 Gene name: mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase Contains complete coding sequence?: Y Chromosome: 14; Localization: 14 q21 Type of DNA: genomic Insert end: Insert end: Insert size (kb): 3.0 Cross references: DNA Seq. Acc.: L36537 Nucleotides 1-2964 of the insert correspond to nucleotides 451-3414 of L36537. Insert lengths(kb): 3.0 Gene product: mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase MGAT2 |
| Insert Size (kb)： | 3.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--3.6, 1.5, 0.86; SalI--3.6, 2.4; XhoI--3.0 (doublet). The insert contains the following restriction sites (approximate kb from the 5 end): NotI--0.38; KpnI--0.50; SalI--0.70; PstI--0.75, 1.57. |
| References： | Tan J, et al. The human UDP-N-acetylglucosamine: alpha-6-D-mannoside-beta-1,2-N-acetylglucosaminyltransferase II gene (MGAT2). Cloning of genomic DNA, localization to chromosome 14q21, expression in insect cells and purification of the recombinant protein. Eur. J. Biochem. 231: 317-328, 1995. PubMed: 7635144 |
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| 产品名称： | pHG30 |
|---|---|
| 商品货号： | TS167957 |
| Designations： | pHG30 |
| GenBank Number： | L36537 |
| Species： | Homo sapiens, human |
| Depositors： | H Schachter |
| Vector： | Construct size (kb): 5.960000038146973 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript II KS+ Intact vector size: 2.961 Type of vector: phagemid Vector end: XhoI Vector end: XhoI Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR replicon: pMB1, f1 Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: MGAT2 Gene name: mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase Contains complete coding sequence?: Y Chromosome: 14; Localization: 14 q21 Type of DNA: genomic Insert end: Insert end: Insert size (kb): 3.0 Cross references: DNA Seq. Acc.: L36537 Nucleotides 1-2964 of the insert correspond to nucleotides 451-3414 of L36537. Insert lengths(kb): 3.0 Gene product: mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase MGAT2 |
| Insert Size (kb)： | 3.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--3.6, 1.5, 0.86; SalI--3.6, 2.4; XhoI--3.0 (doublet). The insert contains the following restriction sites (approximate kb from the 5 end): NotI--0.38; KpnI--0.50; SalI--0.70; PstI--0.75, 1.57. |
| References： | Tan J, et al. The human UDP-N-acetylglucosamine: alpha-6-D-mannoside-beta-1,2-N-acetylglucosaminyltransferase II gene (MGAT2). Cloning of genomic DNA, localization to chromosome 14q21, expression in insect cells and purification of the recombinant protein. Eur. J. Biochem. 231: 317-328, 1995. PubMed: 7635144 |
