| 产品名称： | pDS pDS5 |
|---|---|
| 商品货号： | TS171577 |
| Designations： | pDS pDS5 |
| Depositors： | H Iba |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 5.8499999046325680 DESCRIPTION OF VECTOR: Intact vector size: 5.850 Type of vector: plasmid Cloning sites: EcoRV Polylinker sites: Cloning capacity: 1.8 kb Construction: pN4 (src deleted) Host range: avian cells; Escherichia coli Features (with orientation and position when available): restriction site: SalI coding sequence: 3 env other: splice acceptor site restriction site: EcoRV other: 3 LTR restriction site: SalI marker(s): ampR, -> replicon: pMB1 Vector: pDS (plasmid) Construction: pN4 (src deleted) Marker(s):ampR Construct size (kb): 5.849999904632568 Features: marker(s): ampR other: 3 LTR other: splice acceptor site replicon: pMB1 restriction site: EcoRV restriction site: SalI coding sequence: 3 env |
| Applications： | retroviral expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRV - 5.9; SalI - 3.3, 2.7; HindIII - 5.9. Avian retroviral expression vector derived from Rous sarcoma virus (RSV), requiring vector pRep(A) (ATCC 87702) for recombinant virus production. Replication- competent virus vectors expressing foreign DNA cloned into the EcoRV site can be recovered from the culture fluids after SalI digestion, ligation to SalI digested pRep (A)DNA and transfection into chicken primary cells or appropriate cells |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Murakami M, et al. High-level expression of exogenous genes by replication-competent retrovirus vectors with an internal ribosomal entry site. Gene 202: 23-29, 1997. PubMed: 9427541 Iba H, et al. Transforming potential and growth stimulating activity of the v-fos and c-fos genes carried by avian retrovirus vectors. Oncogene Res. 2: 121-133, 1988. PubMed: 2851123 Yokouchi Y, et al. Misexpression of Hoxa-13 induces cartilage homeotic transformation and changes cell adhesiveness in chick limb buds. Genes Dev. 9: 2509-2522, 1995. PubMed: 7590231 |
| Related Products： | component of:ATCC 87703 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
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| 产品名称： | pDS pDS5 |
|---|---|
| 商品货号： | TS171577 |
| Designations： | pDS pDS5 |
| Depositors： | H Iba |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 5.8499999046325680 DESCRIPTION OF VECTOR: Intact vector size: 5.850 Type of vector: plasmid Cloning sites: EcoRV Polylinker sites: Cloning capacity: 1.8 kb Construction: pN4 (src deleted) Host range: avian cells; Escherichia coli Features (with orientation and position when available): restriction site: SalI coding sequence: 3 env other: splice acceptor site restriction site: EcoRV other: 3 LTR restriction site: SalI marker(s): ampR, -> replicon: pMB1 Vector: pDS (plasmid) Construction: pN4 (src deleted) Marker(s):ampR Construct size (kb): 5.849999904632568 Features: marker(s): ampR other: 3 LTR other: splice acceptor site replicon: pMB1 restriction site: EcoRV restriction site: SalI coding sequence: 3 env |
| Applications： | retroviral expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRV - 5.9; SalI - 3.3, 2.7; HindIII - 5.9. Avian retroviral expression vector derived from Rous sarcoma virus (RSV), requiring vector pRep(A) (ATCC 87702) for recombinant virus production. Replication- competent virus vectors expressing foreign DNA cloned into the EcoRV site can be recovered from the culture fluids after SalI digestion, ligation to SalI digested pRep (A)DNA and transfection into chicken primary cells or appropriate cells |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Murakami M, et al. High-level expression of exogenous genes by replication-competent retrovirus vectors with an internal ribosomal entry site. Gene 202: 23-29, 1997. PubMed: 9427541 Iba H, et al. Transforming potential and growth stimulating activity of the v-fos and c-fos genes carried by avian retrovirus vectors. Oncogene Res. 2: 121-133, 1988. PubMed: 2851123 Yokouchi Y, et al. Misexpression of Hoxa-13 induces cartilage homeotic transformation and changes cell adhesiveness in chick limb buds. Genes Dev. 9: 2509-2522, 1995. PubMed: 7590231 |
| Related Products： | component of:ATCC 87703 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
