| 产品名称： | Chlamydophila abortus |
|---|---|
| 商品货号： | TS172022 |
| Classification： | Chlamydiaceae, Chlamydophila |
| Deposited As： | Ovine chlamydial abortion |
| Agent： | Chlamydophila abortus |
| Strain： | B-577 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Kidney from aborted ovine fetus |
| Product Format： | frozen |
| Storage Conditions： | Vapor phase of liquid nitrogen |
| Images： |  |
| Comments： | Please see ATCC Virology Guide for more detailed information on propagation. Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus. |
| Effect on Host： | Intracellular inclusion bodies visualized by fluorescent staining |
| Recommended Host： | McCoy (ATCC® CRL-1696™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on an 80-100% confluent, 24-48 hour old cellular monolayer. Sonicate seed material for 20 seconds at approximately 240W to disrupt cells.xa0 Prepare dilution of chlamydia in minimum amount of volume (e.g. 1 mL per 25 cm2).xa0 Remove cell growth medium and inoculate with disrupted material. For adsorption, centrifuge at 1500 x g at 25°C for 1 hour.xa0 End adsorption by adding agent growth medium. Incubation:xa0 Incubate infected culture for 72 hours at 37°C in a humidified 5% CO2 atmosphere. Preservation: Controlled-rate freezing. |
| Name of Depositor： | J Storz |
| Source： | Kidney from aborted ovine fetus |
| References： | Storz J. Psittacosis-lymphogranuloma infection of sheep. Antigenic structures and interrelations of PL agents associated with polyarthritis, enzootic abortion, intrauterine and latent intestinal infections. J. Comp. Pathol. 76: 351-362, 1966. PubMed: 6008377 Everett KD, et al. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int. J. Syst. Bacteriol. 49: 415-440, 1999. PubMed: 10319462 type strain |
| Cross References： | Nucleotide (GenBank) : D85709 Chlamydophila abortus DNA for 16S ribosomal RNA. Nucleotide (GenBank) : AB001783 Chlamydophila abortus gene for 16S rRNA, strain B577. Nucleotide (GenBank) : AJ012170 Chlamydophila abortus partial rnpB gene for RNase P RNA subunit. Nucleotide (GenBank) : U68445 Chlamydophila abortus B577 16S ribosomal RNA gene, partial sequence, intergenic spacer, and 23S ribosomal RNA gene, partial sequence. |
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| 产品名称： | Chlamydophila abortus |
|---|---|
| 商品货号： | TS172022 |
| Classification： | Chlamydiaceae, Chlamydophila |
| Deposited As： | Ovine chlamydial abortion |
| Agent： | Chlamydophila abortus |
| Strain： | B-577 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Kidney from aborted ovine fetus |
| Product Format： | frozen |
| Storage Conditions： | Vapor phase of liquid nitrogen |
| Images： | |
| Comments： | Please see ATCC Virology Guide for more detailed information on propagation. Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus. |
| Effect on Host： | Intracellular inclusion bodies visualized by fluorescent staining |
| Recommended Host： | McCoy (ATCC® CRL-1696™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on an 80-100% confluent, 24-48 hour old cellular monolayer. Sonicate seed material for 20 seconds at approximately 240W to disrupt cells.xa0 Prepare dilution of chlamydia in minimum amount of volume (e.g. 1 mL per 25 cm2).xa0 Remove cell growth medium and inoculate with disrupted material. For adsorption, centrifuge at 1500 x g at 25°C for 1 hour.xa0 End adsorption by adding agent growth medium. Incubation:xa0 Incubate infected culture for 72 hours at 37°C in a humidified 5% CO2 atmosphere. Preservation: Controlled-rate freezing. |
| Name of Depositor： | J Storz |
| Source： | Kidney from aborted ovine fetus |
| References： | Storz J. Psittacosis-lymphogranuloma infection of sheep. Antigenic structures and interrelations of PL agents associated with polyarthritis, enzootic abortion, intrauterine and latent intestinal infections. J. Comp. Pathol. 76: 351-362, 1966. PubMed: 6008377 Everett KD, et al. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int. J. Syst. Bacteriol. 49: 415-440, 1999. PubMed: 10319462 type strain |
| Cross References： | Nucleotide (GenBank) : D85709 Chlamydophila abortus DNA for 16S ribosomal RNA. Nucleotide (GenBank) : AB001783 Chlamydophila abortus gene for 16S rRNA, strain B577. Nucleotide (GenBank) : AJ012170 Chlamydophila abortus partial rnpB gene for RNase P RNA subunit. Nucleotide (GenBank) : U68445 Chlamydophila abortus B577 16S ribosomal RNA gene, partial sequence, intergenic spacer, and 23S ribosomal RNA gene, partial sequence. |
