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pZZ2
pZZ2
规格:
货期:
编号:TS172071
品牌:Testobio
产品名称: pZZ2
商品货号: TS172071
Designations: pZZ2
Species: Escherichia coli (Migula) Castellani and Chalmers
Depositors: SJ Elledge
Applications:
mutation detection beta-galactosidase beta-D-galactosidase
Vector:
Construct size (kb): 11.5
Insert:
DNA: genomic
Insert lengths(kb): 0.8999999761581421
Gene product: ribonucleotide reductase (ribonucleoside-diphosphate reductase) large subunit(ribonucleotide reductase, large subunit) RNR3
Target Gene: beta-galactosidase
Insert Size (kb): 0.900
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): BamHI/XhoI--11.0, 0.9; EcoRI--12.0; SalI--12.0.
An RNR3-lacZ transcriptional fusion construct wherein beta-galactosidase expression is regulated by the RNR3 promoter. Includes up to codon 61 of RNR3. Can be used to assay RNR3 activity, such as that induced by DNA damage.
Classification: Enterobacteriaceae, Escherichia
References:

Zhou Z, Elledge SJ. Isolation of crt mutants constitutive for transcription of the DNA damage inducible gene RNR3 in Saccharomyces cerevisiae. Genetics 131: 851-866, 1992. PubMed: 1516817

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pZZ2

  • 货号: TS172071
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  • 品牌 : TESTOBIO
产品名称: pZZ2
商品货号: TS172071
Designations: pZZ2
Species: Escherichia coli (Migula) Castellani and Chalmers
Depositors: SJ Elledge
Applications:
mutation detection beta-galactosidase beta-D-galactosidase
Vector:
Construct size (kb): 11.5
Insert:
DNA: genomic
Insert lengths(kb): 0.8999999761581421
Gene product: ribonucleotide reductase (ribonucleoside-diphosphate reductase) large subunit(ribonucleotide reductase, large subunit) RNR3
Target Gene: beta-galactosidase
Insert Size (kb): 0.900
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): BamHI/XhoI--11.0, 0.9; EcoRI--12.0; SalI--12.0.
An RNR3-lacZ transcriptional fusion construct wherein beta-galactosidase expression is regulated by the RNR3 promoter. Includes up to codon 61 of RNR3. Can be used to assay RNR3 activity, such as that induced by DNA damage.
Classification: Enterobacteriaceae, Escherichia
References:

Zhou Z, Elledge SJ. Isolation of crt mutants constitutive for transcription of the DNA damage inducible gene RNR3 in Saccharomyces cerevisiae. Genetics 131: 851-866, 1992. PubMed: 1516817

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