Babesia microti (Franca) Reichenow

Babesia microti (Franca) Reichenow

规格:

货期:

编号:TS172156

品牌:Testobio

产品名称：	Babesia microti (Franca) Reichenow
商品货号：	TS172156
Deposited As：	Babesia microti
Strain Designations：	Lab Strain 1
Biosafety Level：	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：	Mouse blood, Greenwich, CT, 2004.
Product Format：	frozen
Storage Conditions：	Frozen Cultures: -70°C for 1 week; liquid N₂ vapor for long term storage Freeze-dried Cultures: 2-8°C Live Cultures: See Protocols section for handling information
Genome Sequenced Strain：	Yes
Comments：	Genome sequencing strain
Growth Conditions：	Culture System: In vivo, SCID mouse
Cryopreservation：	Harvest and Preservation Prepare a 30% (v/v) sterile glycerol solution in Alsevers solution. Dispense 0.5 mL of anticoagulant solution into a 15 mL test tube. Add to the anticoagulant blood collected by orbital bleeding from SCID mice that had reached or are near peak parasitemia. Invert the tube several times to mix the blood with the anticoagulant. Mix the heparinized blood with the 30% glycerol solution in a 2:1 ratio.xa0 If any clotting has occurred do not use.xa0 After mixing, the final concentration of cryoprotectant solution will be 10% (v/v).xa0 The mixture should be placed in a 4°C ice bath.xa0 The time from the mixing of the cell preparation and glycerol stock solution before the freezing process is begun should be no less than 15 min and no longer than 30 min. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryovials.xa0 Filled ampules should be placed in a 4°C ice bath.xa0 Do not immerse ampules to the level of the vial cap. Plunge ampules from 4°C into liquid nitrogen.xa0 The frozen preparations may be stored in a mechanical freezer until needed, however storage in either the vapor or liquid phase of a nitrogen refrigerator is recommended for the longest viability. To thaw a frozen ampule, place in a 35°C water bath, until thawed (2-3 min).xa0 Immerse the ampule just sufficient to cover the frozen material.xa0 Do not agitate the ampule. Immediately after thawing, aseptically remove the contents of the ampule with a syringe and inoculate an uninfected SCID mouse.xa0 Follow the protocol for maintenance in vivo.xa0 The course of infection may be longer or shorter than usual depending on recovery of the parasite from the frozen state.
Name of Depositor：	C Ben Mamoun
Chain of Custody：	ATCC
References：	Anderson JF, Magnarelli LA. Babesiosis in Fairfield County, Connecticut. Emerg. Infect. Dis. 10(3): 545-546, 2004. PubMed: 15116708

首页 > 产品中心 > 微生物培养 > 菌株 > null > Babesia microti (Franca) Reichenow

最新活动福利

产品中心 Product Center

联系我们CONTACT US

0574-87917803
24小时服务热线
testobio@163.com
销售邮箱
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址

联系方式

章丽敏
17757489010
朱静
18067106081
李晴
18067408036
李晶晶
13375187189
周雪
13372109301

Babesia microti (Franca) Reichenow

货号: TS172156
好评

询价

品牌 : TESTOBIO

加入购物车在线咨询

产品名称：	Babesia microti (Franca) Reichenow
商品货号：	TS172156
Deposited As：	Babesia microti
Strain Designations：	Lab Strain 1
Biosafety Level：	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：	Mouse blood, Greenwich, CT, 2004.
Product Format：	frozen
Storage Conditions：	Frozen Cultures: -70°C for 1 week; liquid N₂ vapor for long term storage Freeze-dried Cultures: 2-8°C Live Cultures: See Protocols section for handling information
Genome Sequenced Strain：	Yes
Comments：	Genome sequencing strain
Growth Conditions：	Culture System: In vivo, SCID mouse
Cryopreservation：	Harvest and Preservation Prepare a 30% (v/v) sterile glycerol solution in Alsevers solution. Dispense 0.5 mL of anticoagulant solution into a 15 mL test tube. Add to the anticoagulant blood collected by orbital bleeding from SCID mice that had reached or are near peak parasitemia. Invert the tube several times to mix the blood with the anticoagulant. Mix the heparinized blood with the 30% glycerol solution in a 2:1 ratio.xa0 If any clotting has occurred do not use.xa0 After mixing, the final concentration of cryoprotectant solution will be 10% (v/v).xa0 The mixture should be placed in a 4°C ice bath.xa0 The time from the mixing of the cell preparation and glycerol stock solution before the freezing process is begun should be no less than 15 min and no longer than 30 min. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryovials.xa0 Filled ampules should be placed in a 4°C ice bath.xa0 Do not immerse ampules to the level of the vial cap. Plunge ampules from 4°C into liquid nitrogen.xa0 The frozen preparations may be stored in a mechanical freezer until needed, however storage in either the vapor or liquid phase of a nitrogen refrigerator is recommended for the longest viability. To thaw a frozen ampule, place in a 35°C water bath, until thawed (2-3 min).xa0 Immerse the ampule just sufficient to cover the frozen material.xa0 Do not agitate the ampule. Immediately after thawing, aseptically remove the contents of the ampule with a syringe and inoculate an uninfected SCID mouse.xa0 Follow the protocol for maintenance in vivo.xa0 The course of infection may be longer or shorter than usual depending on recovery of the parasite from the frozen state.
Name of Depositor：	C Ben Mamoun
Chain of Custody：	ATCC
References：	Anderson JF, Magnarelli LA. Babesiosis in Fairfield County, Connecticut. Emerg. Infect. Dis. 10(3): 545-546, 2004. PubMed: 15116708

testopic

小红书

扫码关注小红书账号

关注我们

联系电话：0574-87917803

章丽敏：17757489010

朱静：18067106081

李晴：18067408036

李晶晶：13375187189

周雪：13372109301

邮箱：testobio@163.com

地址：浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼

在线留言

备案号：浙ICP备2021006467号-1

合作单位: