| 产品名称： | pHAF2 |
|---|---|
| 商品货号： | TS172801 |
| Designations： | pHAF2 |
| GenBank Number： | J00077 |
| Species： | Homo sapiens, human |
| Depositors： | T Tamaoki |
| Vector： | Construct size (kb): 5.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.8999999761581421 Tissue: embryonal carcinoma Gene product: alpha-fetoprotein( alpha-1-fetoprotein, alpha-foetoprotein, alpha-fetoglobulin) AFP Alleles: B1, B2, C1, C2, D1, D2 |
| Insert Size (kb)： | 0.900 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.4; HindIII--5.4; PstI--4.1, 0.9. Insert covers about 40% of the mRNA sequence (3 end) including coding for domain 2 and all of domain 3 of the protein. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. |
| References： | Morinaga T, et al. Primary structures of human alpha-fetoprotein and its mRNA. Proc. Natl. Acad. Sci. USA 80: 4604-4608, 1983. PubMed: 6192439 Nakao K, et al. c-Ha-ras down regulates the alpha-fetoprotein gene but not the albumin gene in human hepatoma cells. Mol. Cell. Biol. 10: 1461-1469, 1990. PubMed: 1690841 Murray JC, et al. Linkage disequilibrium of ALB and AFP RFLPs predicts 5 to 3 orientation. Cytogenet. Cell Genet. 40: 708, 1985. Morinaga T, et al. Afphafetoprotein messenger RNA in human embryonal carcinoma grown in nude mice, and cloning of its complementary DNA. Oncodev. Biol. Med. 3: 301-313, 1982. PubMed: 6182554 Murray JC, et al. RFLPs for the human alphafetoprotein (AFP), at 4q11-4q13. Nucleic Acids Res. 13: 6794, 1985. PubMed: 2414731 Barbara Weiffenbach, personal communication |
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| 产品名称： | pHAF2 |
|---|---|
| 商品货号： | TS172801 |
| Designations： | pHAF2 |
| GenBank Number： | J00077 |
| Species： | Homo sapiens, human |
| Depositors： | T Tamaoki |
| Vector： | Construct size (kb): 5.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.8999999761581421 Tissue: embryonal carcinoma Gene product: alpha-fetoprotein( alpha-1-fetoprotein, alpha-foetoprotein, alpha-fetoglobulin) AFP Alleles: B1, B2, C1, C2, D1, D2 |
| Insert Size (kb)： | 0.900 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.4; HindIII--5.4; PstI--4.1, 0.9. Insert covers about 40% of the mRNA sequence (3 end) including coding for domain 2 and all of domain 3 of the protein. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. |
| References： | Morinaga T, et al. Primary structures of human alpha-fetoprotein and its mRNA. Proc. Natl. Acad. Sci. USA 80: 4604-4608, 1983. PubMed: 6192439 Nakao K, et al. c-Ha-ras down regulates the alpha-fetoprotein gene but not the albumin gene in human hepatoma cells. Mol. Cell. Biol. 10: 1461-1469, 1990. PubMed: 1690841 Murray JC, et al. Linkage disequilibrium of ALB and AFP RFLPs predicts 5 to 3 orientation. Cytogenet. Cell Genet. 40: 708, 1985. Morinaga T, et al. Afphafetoprotein messenger RNA in human embryonal carcinoma grown in nude mice, and cloning of its complementary DNA. Oncodev. Biol. Med. 3: 301-313, 1982. PubMed: 6182554 Murray JC, et al. RFLPs for the human alphafetoprotein (AFP), at 4q11-4q13. Nucleic Acids Res. 13: 6794, 1985. PubMed: 2414731 Barbara Weiffenbach, personal communication |
