你好,请登录   免费注册    |    收藏本站
联系电话: 0574-87917803
联系电话: call_new 0574-87917803
Plasmodium falciparum Welch
Plasmodium falciparum Welch
规格:
货期:
编号:TS172918
品牌:Testobio
产品名称: Plasmodium falciparum Welch
商品货号: TS172918
Strain Designations: FCR-3/Gambia Subline F-86
Application:
Emerging infectious disease research
Vector borne research
Biosafety Level: 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation:
derived from ATCC 30932
Product Format: frozen
Storage Conditions: Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Type Strain: no
Antibiotic Resistance:
Chloroquine resistant.
Comments:
Selected for knobby trait.
Chloroquine resistant.
pyrimethamine-sensitive
Medium: ATCC® Medium 2196: Malaria medium, complete
Cryopreservation:

Only young cells (rings) can be frozen in glycerolyte medium** because their membranes are more robust.

  1. Centrifuge ring-stage culture for 5 mins. at 1000 x g in 50 ml centrifuge tube.
  2. Aspirate supernatant using sterile Pasteur pipet.
  3. Resuspend pellet gently in remaining supernatant.
  4. Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet dropwise via a syringe as follows:
    1. Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.
    2. Add the remaining 4 volumes of glycerolyte and gently agitate.
  5. Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.).
  6. Plunge vials into liquid nitrogen (-196oC) the next day and store in liquid nitrogen or liquid nitrogen vapor.
Name of Depositor: W Trager
Special Collection: ATCC
References:

Jensen JB, Trager W. Plasmodium falciparum in culture: establishment of additional strains. Am. J. Trop. Med. Hyg. 27: 743-746, 1978. PubMed: 356635

首页 > 产品中心 > 微生物培养 > 菌株 > null > Plasmodium falciparum Welch

Plasmodium falciparum Welch

  • 货号: TS172918
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: Plasmodium falciparum Welch
商品货号: TS172918
Strain Designations: FCR-3/Gambia Subline F-86
Application:
Emerging infectious disease research
Vector borne research
Biosafety Level: 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation:
derived from ATCC 30932
Product Format: frozen
Storage Conditions: Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Type Strain: no
Antibiotic Resistance:
Chloroquine resistant.
Comments:
Selected for knobby trait.
Chloroquine resistant.
pyrimethamine-sensitive
Medium: ATCC® Medium 2196: Malaria medium, complete
Cryopreservation:

Only young cells (rings) can be frozen in glycerolyte medium** because their membranes are more robust.

  1. Centrifuge ring-stage culture for 5 mins. at 1000 x g in 50 ml centrifuge tube.
  2. Aspirate supernatant using sterile Pasteur pipet.
  3. Resuspend pellet gently in remaining supernatant.
  4. Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet dropwise via a syringe as follows:
    1. Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.
    2. Add the remaining 4 volumes of glycerolyte and gently agitate.
  5. Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.).
  6. Plunge vials into liquid nitrogen (-196oC) the next day and store in liquid nitrogen or liquid nitrogen vapor.
Name of Depositor: W Trager
Special Collection: ATCC
References:

Jensen JB, Trager W. Plasmodium falciparum in culture: establishment of additional strains. Am. J. Trop. Med. Hyg. 27: 743-746, 1978. PubMed: 356635

合作单位: