| 产品名称： | Saccharomyces cerevisiae AB320 genomic library in YEp13 Escherichia coli |
|---|---|
| 商品货号： | TS173241 |
| Designations： | Saccharomyces cerevisiae AB320 genomic library in YEp13 Escherichia coli |
| GenBank Definition： | Saccharomyces cerevisiae sec11-7 suppressor Spc1p (SPC1) gene, |
| GenBank Number： | U26257 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | SI Reed, Department of Molecular Biology, Scripps Research Institute |
| Vector： | Name of vector: YEp13 Intact vector size (kb): 10.667 Type of vector: plasmid Cloning sites: BamHI PvuII XhoI Construction: pBR322, S. cerevisiae LEU2, 2 micron ori Host range: S. cerevisiae; E. coli Features: promoter: none marker(s): tetR, →, 89-1279 replicon: pMB1, 2540-2540 marker(s): ampR, <-, 3298-4158 marker(s): LEU2, →, 5843-6937 replicon: 2 micron, →, 8988-10329 Cross references: DNA Seq. Acc.: U03498 Distribution host: Escherichia coli RR1 (ATCC® 31343™) Distributed: Frozen Growth temperature: 37°C |
| Insert： | Type of insert: genomic Insert size: 5-20 kb Type of digest: Sau3A partial Markers: ampR, LEU2 Number of independent recombinants: 8.20x104 Nonrecombinants (%): 50.0 Number of clones for complete coverage: 1.0x104 Titer: 5.9x1010 cfu/mL |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Frozen glycerol stock of Escherichia coli containing the plasmid library |
| Comments： | A Saccharomyces cerevisiae genomic library in plasmid YEp13 |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Nasmyth KA, Reed SI. Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene. Proc. Natl. Acad. Sci. USA 77: 2119-2123, 1980. PubMed: 6246523 Demianova M, et al. Yeast proteins related to the p40/laminin receptor precursor are essential components of the 40 S ribosomal subunit. J. Biol. Chem. 271: 11383-11391, 1996. PubMed: 8626693 |
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| 产品名称： | Saccharomyces cerevisiae AB320 genomic library in YEp13 Escherichia coli |
|---|---|
| 商品货号： | TS173241 |
| Designations： | Saccharomyces cerevisiae AB320 genomic library in YEp13 Escherichia coli |
| GenBank Definition： | Saccharomyces cerevisiae sec11-7 suppressor Spc1p (SPC1) gene, |
| GenBank Number： | U26257 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | SI Reed, Department of Molecular Biology, Scripps Research Institute |
| Vector： | Name of vector: YEp13 Intact vector size (kb): 10.667 Type of vector: plasmid Cloning sites: BamHI PvuII XhoI Construction: pBR322, S. cerevisiae LEU2, 2 micron ori Host range: S. cerevisiae; E. coli Features: promoter: none marker(s): tetR, →, 89-1279 replicon: pMB1, 2540-2540 marker(s): ampR, <-, 3298-4158 marker(s): LEU2, →, 5843-6937 replicon: 2 micron, →, 8988-10329 Cross references: DNA Seq. Acc.: U03498 Distribution host: Escherichia coli RR1 (ATCC® 31343™) Distributed: Frozen Growth temperature: 37°C |
| Insert： | Type of insert: genomic Insert size: 5-20 kb Type of digest: Sau3A partial Markers: ampR, LEU2 Number of independent recombinants: 8.20x104 Nonrecombinants (%): 50.0 Number of clones for complete coverage: 1.0x104 Titer: 5.9x1010 cfu/mL |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Frozen glycerol stock of Escherichia coli containing the plasmid library |
| Comments： | A Saccharomyces cerevisiae genomic library in plasmid YEp13 |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Nasmyth KA, Reed SI. Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene. Proc. Natl. Acad. Sci. USA 77: 2119-2123, 1980. PubMed: 6246523 Demianova M, et al. Yeast proteins related to the p40/laminin receptor precursor are essential components of the 40 S ribosomal subunit. J. Biol. Chem. 271: 11383-11391, 1996. PubMed: 8626693 |
