p414 GALL
p414 GALL
规格:
货期:
编号:TS173380
品牌:Testobio
| 产品名称： | p414 GALL |
|---|---|
| 商品货号： | TS173380 |
| Designations： | p414 GALL |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.444 Vector: p414 GALL (plasmid) Promoters: Promoter for expression GALL Construction: pRS414, GALL promoter Marker(s):TRP1,ampR Construct size (kb): 5.444 Features: marker(s): TRP1 marker(s): ampR promoter for expression: GALL MCS: XhoI...SpeI terminator: CYC1 centromere: CEN6/ARSH4 |
| Applications： | YE-type (episomal) shuttle vector YX-type (expression) shuttle vector vector with low copy number |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 1.9, 0.45; EcoRI--5.4; KpnI--5.4. The galactokinase (GALL) promoter is a deletion variant of GAL1, which lacks one of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed by glucose and is induced at a moderate level by galactose. Low copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736 |
| Related Products： | component of:ATCC 87670 |
| Shipped： | frozen |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
p414 GALL
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | p414 GALL |
|---|---|
| 商品货号： | TS173380 |
| Designations： | p414 GALL |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.444 Vector: p414 GALL (plasmid) Promoters: Promoter for expression GALL Construction: pRS414, GALL promoter Marker(s):TRP1,ampR Construct size (kb): 5.444 Features: marker(s): TRP1 marker(s): ampR promoter for expression: GALL MCS: XhoI...SpeI terminator: CYC1 centromere: CEN6/ARSH4 |
| Applications： | YE-type (episomal) shuttle vector YX-type (expression) shuttle vector vector with low copy number |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 1.9, 0.45; EcoRI--5.4; KpnI--5.4. The galactokinase (GALL) promoter is a deletion variant of GAL1, which lacks one of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed by glucose and is induced at a moderate level by galactose. Low copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736 |
| Related Products： | component of:ATCC 87670 |
| Shipped： | frozen |
