| 产品名称： | phgJE3-3 |
|---|---|
| 商品货号： | TS173560 |
| Designations： | phgJE3-3 |
| GenBank Number： | M26035 |
| Species： | Homo sapiens, human |
| Depositors： | BJ Rollins |
| Vector： | Construct size (kb): 3.799999952316284 |
| Insert： | DNA: genomic Insert lengths(kb): 0.800000011920929 Tissue: embryonic lung fibroblast WI-38 (serum-induced) Gene product: cytokine A2, small inducible( monocyte chemotactic protein, monocyte chemotactic protein 1, homologous to mouse Sig-je, small inducible cytokine A2, small inducible cytokine subfamily A (Cys-Cys), member 2, small inducible gene JE) JE* |
| Insert Size (kb)： | 0.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains the following sites (with nucleotide positions according to the published sequence): NaeI at -426; HindIII at -420; SacI at -408 and PstI at -307. Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.8; HindIII--3.65, 0.15; BamHI--3.8; PstI--3.8; XbaI/SacI--2.9, 0.7, 0.2. The 5 clone phgJE3-3 (TS173560/61367) and the 3 clone phgJE2-1 (ATCC 61368/61369) together generate a complete gene. This insert was subcloned from lambdahJE-7 and extends from -531 relative to the mRNA cap site into the first intron which begins as +228. It is oriented in this clone with transcription from the SP6 promoter giving the + strand. |
| References： | Rollins BJ, et al. The human homolog of the JE gene encodes a monocyte secretory protein. Mol. Cell. Biol. 9: 4687-4695, 1989. PubMed: 2513477 Barrett J Rollins, personal communication |
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| 产品名称： | phgJE3-3 |
|---|---|
| 商品货号： | TS173560 |
| Designations： | phgJE3-3 |
| GenBank Number： | M26035 |
| Species： | Homo sapiens, human |
| Depositors： | BJ Rollins |
| Vector： | Construct size (kb): 3.799999952316284 |
| Insert： | DNA: genomic Insert lengths(kb): 0.800000011920929 Tissue: embryonic lung fibroblast WI-38 (serum-induced) Gene product: cytokine A2, small inducible( monocyte chemotactic protein, monocyte chemotactic protein 1, homologous to mouse Sig-je, small inducible cytokine A2, small inducible cytokine subfamily A (Cys-Cys), member 2, small inducible gene JE) JE* |
| Insert Size (kb)： | 0.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains the following sites (with nucleotide positions according to the published sequence): NaeI at -426; HindIII at -420; SacI at -408 and PstI at -307. Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.8; HindIII--3.65, 0.15; BamHI--3.8; PstI--3.8; XbaI/SacI--2.9, 0.7, 0.2. The 5 clone phgJE3-3 (TS173560/61367) and the 3 clone phgJE2-1 (ATCC 61368/61369) together generate a complete gene. This insert was subcloned from lambdahJE-7 and extends from -531 relative to the mRNA cap site into the first intron which begins as +228. It is oriented in this clone with transcription from the SP6 promoter giving the + strand. |
| References： | Rollins BJ, et al. The human homolog of the JE gene encodes a monocyte secretory protein. Mol. Cell. Biol. 9: 4687-4695, 1989. PubMed: 2513477 Barrett J Rollins, personal communication |
