Babesia microti (Franca) Reichenow

Babesia microti (Franca) Reichenow

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编号:TS173838

品牌:Testobio

产品名称：	Babesia microti (Franca) Reichenow
商品货号：	TS173838
Deposited As：	Babesia microti
Strain Designations：	Nan-Hs-2011 (N11-50)
Biosafety Level：	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：	Blood, human babesiosis, Nantucket, MA, 2010
Product Format：	frozen
Storage Conditions：	Frozen Cultures: -70°C for 1 week; liquid N₂ vapor for long term storage Freeze-dried Cultures: 2-8°C Live Cultures: See Protocols section for handling information
Genome Sequenced Strain：	Yes
Comments：	Genome strain
Growth Conditions：	Culture System: In vivo, Golden Syrian hamster
Cryopreservation：	Reagents Alsevers Solution NaCl, 4.2 g Na₃citrate•2H₂O, 8.0 g Glucose, 20.5 g Glass distilled H₂O to 1.0 L Dissolve components in glass distilled H₂O, adjust the pH to 6.1 with 10% (w/v) citric acid and filter sterilize. The solution can be obtained from Sigma-Aldrich (cat# A3551). Harvest and Preservation* Prepare a 30% (v/v) sterile glycerol solution in Alsevers solution. Draw approximately 0.5 mL of anticoagulant solution (Yaegers or heparin, etc.) into a syringe and move it back and forth over the length of the syringe, several times.xa0 Remove all air bubbles.xa0 Draw blood by cardiac puncture into the syringe from a host animal that has reached or is near peak parasitemia.xa0 If clotting occurs during extraction of blood, insufficient heparin was used.xa0xa0 Mix the heparinized blood with the 30% glycerol solution in a 2:1 ratio.xa0 If any clotting has occurred do not use.xa0 After mixing, the final concentration of cryoprotectant solution will be 10% (v/v).xa0 The mixture should be placed in a 4°C ice bath.xa0 The time from the mixing of the cell preparation and glycerol stock solution before the freezing process is begun should be no less than 15 min and no longer than 30 min. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryovials (special plastic vials for cryopreservation).xa0 Filled ampules should be placed in a 4°C ice bath.xa0 Do not immerse ampules to the level of the vial cap. Plunge ampules from 4°C into liquid nitrogen.xa0 The frozen preparations may be stored in a mechanical freezer until needed, however storage in either the vapor or liquid phase of a nitrogen refrigerator is recommended for the longest viability. To thaw a frozen ampule, place in a 35°C water bath, until thawed (2-3 min). xa0Immerse the ampule just sufficient to cover the frozen material.xa0 Do not agitate the ampule. Immediately after thawing, aseptically remove the contents of the ampule with a syringe and inoculate an uninfected hamster.xa0 Follow the protocol for maintenance in vivo.xa0 The course of infection may be longer or shorter than usual depending on recovery of the parasite from the frozen state.
Name of Depositor：	C. Ben Mamoun
Chain of Custody：	ATCC
Cross References：	Nucleotide (GenBank) : PRJNA157393 Babesia microti genome sequencing project

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Babesia microti (Franca) Reichenow

货号: TS173838
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产品名称：	Babesia microti (Franca) Reichenow
商品货号：	TS173838
Deposited As：	Babesia microti
Strain Designations：	Nan-Hs-2011 (N11-50)
Biosafety Level：	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：	Blood, human babesiosis, Nantucket, MA, 2010
Product Format：	frozen
Storage Conditions：	Frozen Cultures: -70°C for 1 week; liquid N₂ vapor for long term storage Freeze-dried Cultures: 2-8°C Live Cultures: See Protocols section for handling information
Genome Sequenced Strain：	Yes
Comments：	Genome strain
Growth Conditions：	Culture System: In vivo, Golden Syrian hamster
Cryopreservation：	Reagents Alsevers Solution NaCl, 4.2 g Na₃citrate•2H₂O, 8.0 g Glucose, 20.5 g Glass distilled H₂O to 1.0 L Dissolve components in glass distilled H₂O, adjust the pH to 6.1 with 10% (w/v) citric acid and filter sterilize. The solution can be obtained from Sigma-Aldrich (cat# A3551). Harvest and Preservation* Prepare a 30% (v/v) sterile glycerol solution in Alsevers solution. Draw approximately 0.5 mL of anticoagulant solution (Yaegers or heparin, etc.) into a syringe and move it back and forth over the length of the syringe, several times.xa0 Remove all air bubbles.xa0 Draw blood by cardiac puncture into the syringe from a host animal that has reached or is near peak parasitemia.xa0 If clotting occurs during extraction of blood, insufficient heparin was used.xa0xa0 Mix the heparinized blood with the 30% glycerol solution in a 2:1 ratio.xa0 If any clotting has occurred do not use.xa0 After mixing, the final concentration of cryoprotectant solution will be 10% (v/v).xa0 The mixture should be placed in a 4°C ice bath.xa0 The time from the mixing of the cell preparation and glycerol stock solution before the freezing process is begun should be no less than 15 min and no longer than 30 min. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryovials (special plastic vials for cryopreservation).xa0 Filled ampules should be placed in a 4°C ice bath.xa0 Do not immerse ampules to the level of the vial cap. Plunge ampules from 4°C into liquid nitrogen.xa0 The frozen preparations may be stored in a mechanical freezer until needed, however storage in either the vapor or liquid phase of a nitrogen refrigerator is recommended for the longest viability. To thaw a frozen ampule, place in a 35°C water bath, until thawed (2-3 min). xa0Immerse the ampule just sufficient to cover the frozen material.xa0 Do not agitate the ampule. Immediately after thawing, aseptically remove the contents of the ampule with a syringe and inoculate an uninfected hamster.xa0 Follow the protocol for maintenance in vivo.xa0 The course of infection may be longer or shorter than usual depending on recovery of the parasite from the frozen state.
Name of Depositor：	C. Ben Mamoun
Chain of Custody：	ATCC
Cross References：	Nucleotide (GenBank) : PRJNA157393 Babesia microti genome sequencing project

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