lambdaSK27 [clone 193]
lambdaSK27 [clone 193]
规格:
货期:
编号:TS174096
品牌:Testobio
| 产品名称： | lambdaSK27 clone 193 |
|---|---|
| 商品货号： | TS174096 |
| Designations： | lambdaSK27 clone 193 |
| Depositors： | ER Zabarovsky, G Winberg, G Klein |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 47.7999992370605500 Vector: lambdaSK27 (phage, lambda - replacement) Marker(s):red+ gam+ Construct size (kb): 47.79999923706055 Features: insert detection: Spi+ marker(s): red+ gam+ replicon: lambda, pMB1, M13 |
| Applications： | vector containing primer sites useful for sequencing vector for constructing genomic libraries vector for other uses vector permitting positive selection for inserts |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--25.0, 13.0, 9.8; EcoRI--24.0 (doublet); SalI--22.0, 9.0, 8.4, 4.4, 4.2. Vector permitting positive selection (Spi) of recombinants. Useful for generating jumping and linking libraries and conversion to phagemid (without selection). Permits construction using partial filling-in. The restriction sites to be used for conversion to phagemid are: BspMII SalI ClaI. The sizes of the major components are (kb): arms--20.3, 9.2; stuffer fragment--13.7; phagemid segments--3.9 (longer arm), 0.7. The order of the major features in this plasmid is: arm - ClaI BspMII SalI - pMB1 ori - ampR - M13 ori - SacI/MCS/SalI - stuffer - SalI/MCS/NotI - ClaI SalI BspMII - arm. |
| References： | Zabarovsky ER, et al. Lambda SK diphasmids: phage lambda vectors for genomic, jumping, linking and cDNA libraries. Gene 127: 1-14, 1993. PubMed: 8486275 Zabarovsky ER, Winberg GN. Improved methods and new vectors for creating libraries of cloned genes. Sov. Sci. Rev. 11: 1-65, 1992. |
| Shipped： | freeze-dried |
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lambdaSK27 [clone 193]
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| 产品名称： | lambdaSK27 clone 193 |
|---|---|
| 商品货号： | TS174096 |
| Designations： | lambdaSK27 clone 193 |
| Depositors： | ER Zabarovsky, G Winberg, G Klein |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 47.7999992370605500 Vector: lambdaSK27 (phage, lambda - replacement) Marker(s):red+ gam+ Construct size (kb): 47.79999923706055 Features: insert detection: Spi+ marker(s): red+ gam+ replicon: lambda, pMB1, M13 |
| Applications： | vector containing primer sites useful for sequencing vector for constructing genomic libraries vector for other uses vector permitting positive selection for inserts |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--25.0, 13.0, 9.8; EcoRI--24.0 (doublet); SalI--22.0, 9.0, 8.4, 4.4, 4.2. Vector permitting positive selection (Spi) of recombinants. Useful for generating jumping and linking libraries and conversion to phagemid (without selection). Permits construction using partial filling-in. The restriction sites to be used for conversion to phagemid are: BspMII SalI ClaI. The sizes of the major components are (kb): arms--20.3, 9.2; stuffer fragment--13.7; phagemid segments--3.9 (longer arm), 0.7. The order of the major features in this plasmid is: arm - ClaI BspMII SalI - pMB1 ori - ampR - M13 ori - SacI/MCS/SalI - stuffer - SalI/MCS/NotI - ClaI SalI BspMII - arm. |
| References： | Zabarovsky ER, et al. Lambda SK diphasmids: phage lambda vectors for genomic, jumping, linking and cDNA libraries. Gene 127: 1-14, 1993. PubMed: 8486275 Zabarovsky ER, Winberg GN. Improved methods and new vectors for creating libraries of cloned genes. Sov. Sci. Rev. 11: 1-65, 1992. |
| Shipped： | freeze-dried |
