| 产品名称： | pB12BSM |
|---|---|
| 商品货号： | TS174123 |
| Designations： | pB12BSM |
| Species： | Beet necrotic yellow vein virus |
| Depositors： | KE Richards |
| Vector： | Construct size (kb): 5.019999980926514 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.820000052452087 Gene product: genome, partial |
| Insert Size (kb)： | 1.820 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI--5.0; PvuII--3.0, 1.7. To synthesize antisense RNA in vitro, cut with EcoRI and transcribe from vector T3 promoter. The insert is nt 4740-6560 of RNA-1 of beet necrotic yellow vein virus isolate F3 from Chenopodium quinoa and is noninfectious. |
| Classification： | Furovirus |
| References： | Bouzoubaa S, et al. Nucleotide sequence of beet necrotic yellow vein virus RNA-1. J. Gen. Virol. 68: 615-626, 1987. Bouzoubaa S, et al. Shortened forms of beet necrotic yellow vein virus RNA-3 and RNA-4: internal deletions and a subgenomic RNA. J. Gen. Virol. 72: 259-266, 1991. PubMed: 1993870 Ken E Richards, personal communication |
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| 产品名称： | pB12BSM |
|---|---|
| 商品货号： | TS174123 |
| Designations： | pB12BSM |
| Species： | Beet necrotic yellow vein virus |
| Depositors： | KE Richards |
| Vector： | Construct size (kb): 5.019999980926514 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.820000052452087 Gene product: genome, partial |
| Insert Size (kb)： | 1.820 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI--5.0; PvuII--3.0, 1.7. To synthesize antisense RNA in vitro, cut with EcoRI and transcribe from vector T3 promoter. The insert is nt 4740-6560 of RNA-1 of beet necrotic yellow vein virus isolate F3 from Chenopodium quinoa and is noninfectious. |
| Classification： | Furovirus |
| References： | Bouzoubaa S, et al. Nucleotide sequence of beet necrotic yellow vein virus RNA-1. J. Gen. Virol. 68: 615-626, 1987. Bouzoubaa S, et al. Shortened forms of beet necrotic yellow vein virus RNA-3 and RNA-4: internal deletions and a subgenomic RNA. J. Gen. Virol. 72: 259-266, 1991. PubMed: 1993870 Ken E Richards, personal communication |
