pRS173
pRS173
规格:
货期:
编号:TS174234
品牌:Testobio
| 产品名称： | pRS173 |
|---|---|
| 商品货号： | TS174234 |
| Designations： | pRS173 |
| Depositors： | P Hieter |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.8000001907348630 Vector: pRS173 (phagemid) Promoters: Promoter T3 Construction: pRSS56 pBluescript KS+, pBS(+), GAL1/10 promoter Marker(s):URA3,ampR Construct size (kb): 5.800000190734863 Features: insert detection: lacZ marker(s): ampR, URA3 promoter: lac, T3, T7, GAL10 replicon: pMB1, f1, ARS1 |
| Applications： | YC-type (centromeric) shuttle vector YX-type (expression) shuttle vector expression vector shuttle vector vector containing primer sites useful for sequencing vector permitting RNA synthesis in vitro vector permitting construction of fusion proteins vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.6; BamHI--6.6; SalI--6.6. Expression is inducible by galactose. YX-type expression shuttle vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis and encodes the lacZ alpha (lacZ) peptide. Transcription from the GAL10 promoter (ATG is included in the sequence) permits the synthesis of a fusion protein (starting with 26 aa of GAL10) through the polylinker in the same frame as lacZ. pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). A fragment containing the URA3 gene was inserted into the NdeI site and a DraIII/EcoRI fragment containing the GAL1 and GAL10 promoters was inserted into the Asp718 site of the MCS of pRSS56. All ends were blunted. The order of the major features in this plasmid is: URA3 - f1 ori (NaeI) - T7 promoter - lacZ/MCS - T3 promoter - GAL10 promoter - GAL1 promoter - pMB1 ori - bla - CEN - ARS1. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Philip Hieter, personal communication |
| Related Products： | component of:ATCC 87054 |
| Shipped： | freeze-dried |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
pRS173
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | pRS173 |
|---|---|
| 商品货号： | TS174234 |
| Designations： | pRS173 |
| Depositors： | P Hieter |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.8000001907348630 Vector: pRS173 (phagemid) Promoters: Promoter T3 Construction: pRSS56 pBluescript KS+, pBS(+), GAL1/10 promoter Marker(s):URA3,ampR Construct size (kb): 5.800000190734863 Features: insert detection: lacZ marker(s): ampR, URA3 promoter: lac, T3, T7, GAL10 replicon: pMB1, f1, ARS1 |
| Applications： | YC-type (centromeric) shuttle vector YX-type (expression) shuttle vector expression vector shuttle vector vector containing primer sites useful for sequencing vector permitting RNA synthesis in vitro vector permitting construction of fusion proteins vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.6; BamHI--6.6; SalI--6.6. Expression is inducible by galactose. YX-type expression shuttle vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis and encodes the lacZ alpha (lacZ) peptide. Transcription from the GAL10 promoter (ATG is included in the sequence) permits the synthesis of a fusion protein (starting with 26 aa of GAL10) through the polylinker in the same frame as lacZ. pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). A fragment containing the URA3 gene was inserted into the NdeI site and a DraIII/EcoRI fragment containing the GAL1 and GAL10 promoters was inserted into the Asp718 site of the MCS of pRSS56. All ends were blunted. The order of the major features in this plasmid is: URA3 - f1 ori (NaeI) - T7 promoter - lacZ/MCS - T3 promoter - GAL10 promoter - GAL1 promoter - pMB1 ori - bla - CEN - ARS1. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Philip Hieter, personal communication |
| Related Products： | component of:ATCC 87054 |
| Shipped： | freeze-dried |
