| 产品名称： | Vaccinia virus ts mutant |
|---|---|
| 商品货号： | TS174330 |
| Classification： | Poxviridae, Orthopoxvirus |
| Deposited As： | Vaccinia virus, Orthopoxvirus |
| Agent： | Vaccinia virus ts mutant |
| Strain： | IHD-W Dts41 |
| Applications： | The mutation was assigned by complementation analysis to vaccinia map location E(2-8). |
| Biosafety Level： | 2 Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses refCDC BMBL recommends vaccination
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | This conditional lethal mutant, originally designated plaque isolate no. 6606, was isolated from the IHD-W strain of Vaccinia by S. Dales. The IHD-W strain was derived from an IHD-J strain of Vaccinia. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5oC. This mutant was assigned a Dales EM category of P, characterized by immature normal particles and mature particles with cores that are predominantly lucent at the center. The mutation was assigned by complementation analysis to vaccinia map location E(2-8). |
| Effect on Host： | Cytopathic effects include enlargement and rounding of cells, followed by death and eventual sloughing. CPE: enlargement, plaques with rounding and eventual sloughing |
| Growth Medium： | ATCC® Medium 30-2003: Eagles Minimum Essential Medium (EMEM) Fetal Bovine Serum |
| Recommended Host： | Production Host: BSC-40 (ATCC CRL-2761) Recommended Host: BSC-40 (ATCC CRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2 |
| Growth Conditions： | 1-3 days at 31 C with 5% CO2 |
| Name of Depositor： | S Dales, RC Condit |
| Special Collection： | NCRR Contract |
| Chain of Custody： | |
| References： | Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807 Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746 Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570 Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |
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| 产品名称： | Vaccinia virus ts mutant |
|---|---|
| 商品货号： | TS174330 |
| Classification： | Poxviridae, Orthopoxvirus |
| Deposited As： | Vaccinia virus, Orthopoxvirus |
| Agent： | Vaccinia virus ts mutant |
| Strain： | IHD-W Dts41 |
| Applications： | The mutation was assigned by complementation analysis to vaccinia map location E(2-8). |
| Biosafety Level： | 2 Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses refCDC BMBL recommends vaccination
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | This conditional lethal mutant, originally designated plaque isolate no. 6606, was isolated from the IHD-W strain of Vaccinia by S. Dales. The IHD-W strain was derived from an IHD-J strain of Vaccinia. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5oC. This mutant was assigned a Dales EM category of P, characterized by immature normal particles and mature particles with cores that are predominantly lucent at the center. The mutation was assigned by complementation analysis to vaccinia map location E(2-8). |
| Effect on Host： | Cytopathic effects include enlargement and rounding of cells, followed by death and eventual sloughing. CPE: enlargement, plaques with rounding and eventual sloughing |
| Growth Medium： | ATCC® Medium 30-2003: Eagles Minimum Essential Medium (EMEM) Fetal Bovine Serum |
| Recommended Host： | Production Host: BSC-40 (ATCC CRL-2761) Recommended Host: BSC-40 (ATCC CRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2 |
| Growth Conditions： | 1-3 days at 31 C with 5% CO2 |
| Name of Depositor： | S Dales, RC Condit |
| Special Collection： | NCRR Contract |
| Chain of Custody： | |
| References： | Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807 Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746 Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570 Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |
