| 产品名称： | Human adenovirus 37 |
|---|---|
| 商品货号： | TS174576 |
| Classification： | Adenoviridae, Mastadenovirus, Human adenovirus D |
| Deposited As： | Adenovirus type 37 |
| Agent： | Human adenovirus 37 |
| Strain： | GW (76-19026) |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Good immunogen in rabbits and horses Cross-reacts with Human adenovirus 10, 13, 19 and 30 by serum neutralization and HAI HA with rat, rhesus monkey and human O type red blood cells |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | WI-38 (ATCC® CCL-75™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 75-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere while rocking continuously. End adsorption by adding virus growth medium. Incubation: Incubate infected culture for 2-11 days at 37°C in a humidified 5% CO2 atmosphere, until CPE are well advance through 90% of the culture. |
| Name of Depositor： | JC de Jong, National Institute for Public Health and the Environment, Netherlands |
| Source： | Human eye with keratoconjunctivitis (possible genitourinary disorder), Netherlands, 1976 |
| Year of Origin： | 1976 |
| References： | Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904 de Jong JC, et al. Adenovirus 37: identification and characterization of a medically important new adenovirus type of subgroup D. J. Med. Virol. 7: 105-118, 1981. PubMed: 7264611 Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016 Wadell G, et al. Characterization of candidate adenovirus 37 by SDS-polyacrylamide gel electrophoresis of virion polypeptides and DNA restriction site mapping. J. Med. Virol. 7: 119-125, 1981. PubMed: 6267186 |
| Cross References： | Nucleotide (GenBank) : U52553 Human adenovirus type 37 virus-associated RNA pre-terminal protein (pTP) and 52,55K protein genes, partial cds. Nucleotide (GenBank) : AF086569 Human adenovirus type 37 early E3B 10.4 kDa protein, early E3B 14.5 kDa protein, and early E3B 14.7 kDa protein genes, complete cds. |
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| 产品名称： | Human adenovirus 37 |
|---|---|
| 商品货号： | TS174576 |
| Classification： | Adenoviridae, Mastadenovirus, Human adenovirus D |
| Deposited As： | Adenovirus type 37 |
| Agent： | Human adenovirus 37 |
| Strain： | GW (76-19026) |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Good immunogen in rabbits and horses Cross-reacts with Human adenovirus 10, 13, 19 and 30 by serum neutralization and HAI HA with rat, rhesus monkey and human O type red blood cells |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | WI-38 (ATCC® CCL-75™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 75-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere while rocking continuously. End adsorption by adding virus growth medium. Incubation: Incubate infected culture for 2-11 days at 37°C in a humidified 5% CO2 atmosphere, until CPE are well advance through 90% of the culture. |
| Name of Depositor： | JC de Jong, National Institute for Public Health and the Environment, Netherlands |
| Source： | Human eye with keratoconjunctivitis (possible genitourinary disorder), Netherlands, 1976 |
| Year of Origin： | 1976 |
| References： | Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904 de Jong JC, et al. Adenovirus 37: identification and characterization of a medically important new adenovirus type of subgroup D. J. Med. Virol. 7: 105-118, 1981. PubMed: 7264611 Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016 Wadell G, et al. Characterization of candidate adenovirus 37 by SDS-polyacrylamide gel electrophoresis of virion polypeptides and DNA restriction site mapping. J. Med. Virol. 7: 119-125, 1981. PubMed: 6267186 |
| Cross References： | Nucleotide (GenBank) : U52553 Human adenovirus type 37 virus-associated RNA pre-terminal protein (pTP) and 52,55K protein genes, partial cds. Nucleotide (GenBank) : AF086569 Human adenovirus type 37 early E3B 10.4 kDa protein, early E3B 14.5 kDa protein, and early E3B 14.7 kDa protein genes, complete cds. |
