| 产品名称： | YRp14-his3delta200 YRp14/his3-delta200, p510 |
|---|---|
| 商品货号： | TS174628 |
| Designations： | YRp14-his3delta200 YRp14/his3-delta200, p510 |
| Depositors： | P Hieter |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 11.1999998092651400 DESCRIPTION OF VECTOR: Intact vector size: 11.200 Type of vector: plasmid Cloning sites: Polylinker sites: Construction: YRp14 Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): ampR, URA3, SUP11 replicon: pMB1, ARS1 Vector: YRp14-his3delta200 (plasmid) Construction: YRp14 Marker(s):URA3,ampR,SUP11 Construct size (kb): 11.19999980926514 Features: marker(s): ampR, URA3, SUP11 replicon: pMB1, ARS1 |
| Applications： | vector for other uses |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--11.0, 0.5; PstI--6.1, 4.0, 1.2; BglII--10.0, 1.15; KpnI--11.5. A marker addition/transplacement vector. Non-integrants grow poorly selecting for Ura3+ (pinpoints at 5 days) while integrants grow well because the SUP11 copy number is maintained at one. If there are no integrants on a transformation plate, streak onto -URA plates and pick papillae. A 1.0 kb region that encompasses the entire coding region of HIS3 was deleted from a genomic EcoRI/SalI fragment (final size=5.1 kb) cloned into the EcoRI/SalI sites of YRp14. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436 Fasullo MT, Davis RW. Direction of chhromosome rearrangements in Saccharomyces cerevisiae by use of his3 recombinational studies. Mol. Cell. Biol. 8: 4370-4380, 1988. PubMed: 3054515 Philip Hieter, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
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| 产品名称： | YRp14-his3delta200 YRp14/his3-delta200, p510 |
|---|---|
| 商品货号： | TS174628 |
| Designations： | YRp14-his3delta200 YRp14/his3-delta200, p510 |
| Depositors： | P Hieter |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 11.1999998092651400 DESCRIPTION OF VECTOR: Intact vector size: 11.200 Type of vector: plasmid Cloning sites: Polylinker sites: Construction: YRp14 Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): ampR, URA3, SUP11 replicon: pMB1, ARS1 Vector: YRp14-his3delta200 (plasmid) Construction: YRp14 Marker(s):URA3,ampR,SUP11 Construct size (kb): 11.19999980926514 Features: marker(s): ampR, URA3, SUP11 replicon: pMB1, ARS1 |
| Applications： | vector for other uses |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--11.0, 0.5; PstI--6.1, 4.0, 1.2; BglII--10.0, 1.15; KpnI--11.5. A marker addition/transplacement vector. Non-integrants grow poorly selecting for Ura3+ (pinpoints at 5 days) while integrants grow well because the SUP11 copy number is maintained at one. If there are no integrants on a transformation plate, streak onto -URA plates and pick papillae. A 1.0 kb region that encompasses the entire coding region of HIS3 was deleted from a genomic EcoRI/SalI fragment (final size=5.1 kb) cloned into the EcoRI/SalI sites of YRp14. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436 Fasullo MT, Davis RW. Direction of chhromosome rearrangements in Saccharomyces cerevisiae by use of his3 recombinational studies. Mol. Cell. Biol. 8: 4370-4380, 1988. PubMed: 3054515 Philip Hieter, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
