| 产品名称： | pLS211 1011 |
|---|---|
| 商品货号： | TS174715 |
| Designations： | pLS211 1011 |
| Species： | Streptococcus pneumoniae (Klein) Chester |
| Depositors： | Brookhaven National Laboratory, SA Lacks, Brookhaven National Laboratory |
| Applications： | produces protein DpnII DNA methylase produces protein DpnII restriction endonuclease |
| Vector： | Construct size (kb): 8.600000381469727 |
| Insert： | DNA: genomic Insert lengths(kb): 3.5 Gene product: DpnII restriction endonuclease dpnA Target Gene: DpnII DNA methylase, DpnII restriction endonuclease |
| Insert Size (kb)： | 3.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.0, 2.7, 1.93; BamHI--5.1, 3.5; BglI--6.1, 2.48; PstI--5.0, 2.4, 1.2. According to the published sequence, the limits of the genes are as follows: dpnM from 441-1295, dpnA from 1285 to 2091, and dpnB from 2078 to 2944. The host strain expresses T7 RNA polymerase, so that very large amounts of DpnII gene products are produced. This was constructed by ligating a 3.5 kb NcoI/BstEII fragment from pLS202 to the 5.1 kb BstEII/NcoI fragment from pLS21 (ATCC 67492). |
| References： | Lacks SA. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of Streptococcus pneumoniae. US Patent 4,960,707 dated Oct 2 1990 Lacks SA, et al. Genetic basis of the complementary DpnI and DpnII restriction systems of S. pneumoniae: an intracellular cassette mechanism. Cell 46: 993-1000, 1986. PubMed: 3019562 de la Campa AG, et al. Protein encoded by the DpnII restriction gene cassette. Two methylases and an endonuclease. J. Mol. Biol. 196: 457-469, 1987. PubMed: 2824782 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pLS211 1011 |
|---|---|
| 商品货号： | TS174715 |
| Designations： | pLS211 1011 |
| Species： | Streptococcus pneumoniae (Klein) Chester |
| Depositors： | Brookhaven National Laboratory, SA Lacks, Brookhaven National Laboratory |
| Applications： | produces protein DpnII DNA methylase produces protein DpnII restriction endonuclease |
| Vector： | Construct size (kb): 8.600000381469727 |
| Insert： | DNA: genomic Insert lengths(kb): 3.5 Gene product: DpnII restriction endonuclease dpnA Target Gene: DpnII DNA methylase, DpnII restriction endonuclease |
| Insert Size (kb)： | 3.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.0, 2.7, 1.93; BamHI--5.1, 3.5; BglI--6.1, 2.48; PstI--5.0, 2.4, 1.2. According to the published sequence, the limits of the genes are as follows: dpnM from 441-1295, dpnA from 1285 to 2091, and dpnB from 2078 to 2944. The host strain expresses T7 RNA polymerase, so that very large amounts of DpnII gene products are produced. This was constructed by ligating a 3.5 kb NcoI/BstEII fragment from pLS202 to the 5.1 kb BstEII/NcoI fragment from pLS21 (ATCC 67492). |
| References： | Lacks SA. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of Streptococcus pneumoniae. US Patent 4,960,707 dated Oct 2 1990 Lacks SA, et al. Genetic basis of the complementary DpnI and DpnII restriction systems of S. pneumoniae: an intracellular cassette mechanism. Cell 46: 993-1000, 1986. PubMed: 3019562 de la Campa AG, et al. Protein encoded by the DpnII restriction gene cassette. Two methylases and an endonuclease. J. Mol. Biol. 196: 457-469, 1987. PubMed: 2824782 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
