你好，请登录   免费注册    |    收藏本站
联系电话: 0574-87917803
你好,请登录   免费注册 | 收藏
联系电话: call_new 0574-87917803
热门搜索: 金黄色葡萄球菌 铜绿假单胞菌 红菇 香菇
Ankistrodesmus braunii Brunnthaler
Ankistrodesmus braunii Brunnthaler
规格:
货期:
编号:TS174773
品牌:Testobio
产品名称： Ankistrodesmus braunii Brunnthaler
商品货号： TS174773
Deposited As： Ankistrodesmus falcatus var. duplex
Strain Designations： UTEX 187 CCAP-202/9a
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Material collected by T. Christensen, Madingley Brick Pits, England, 1949
Product Format： frozen
Storage Conditions： Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain： no
Comments：
photosynthetic
Medium： ATCC® Medium 847: Algal proteose agar
Growth Conditions：
Temperature: 25°C
Culture System: Axenic
Cryopreservation： Harvest and Preservation
  1. Harvest cells from a culture that is at or near peak density by centrifugation at 700-800 x g for 5 min.
  2. Adjust the concentration of cells to 2 x 106 - 2 x 107/mL in fresh medium.
  3. While cells are centrifuging prepare a 14% (v/v) solution of sterile DMSO in fresh medium.
  4. Mix the cell preparation and the 14% DMSO solution in equal portions. Thus, the final concentration will be 106 - 107 cells/mL and 7% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution to the beginning of the freezing process should be no less than 5 min and no greater than 15 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the vials in a controlled rate freezing unit.xa0 From room temperature cool at -1°C/min to -40°C.xa0 If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion.xa0 At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0
  7. The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C.
  8. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial.
  9. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and add to a 16 x 125 mm screw-capped test tube containing 5 mL of ATCC medium 847 broth or to the surface of an ATCC medium 847 agar plate (20 x 100 mm Petri plate containing 20 mL of ATCC medium 5 agar).
  10. Incubate the culture at 50-100 µEinsteins/m2/s irradiance at 25°C.xa0 Maintain under a 14/10h light-dark photoperiod.
Name of Depositor： UTEX
Chain of Custody：
ATCC <-- UTEX <-- E A George
Year of Origin： 1949
References：

Starr RC. The culture collection of algae at Indiana University. Am. J. Bot. 51: 1013-1044, 1964.

首页 > 产品中心 > 微生物培养 > 菌株 > null > Ankistrodesmus braunii Brunnthaler

产品中心 Product Center

联系我们CONTACT US

  • 0574-87917803
    24小时服务热线
  • testobio@163.com
    销售邮箱
  • 浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
    地址

联系方式

  • 章丽敏
    17757489010
  • 朱静
    18067106081
  • 李晴
    18067408036
  • 李晶晶
    13375187189
  • 周雪
    13372109301

Ankistrodesmus braunii Brunnthaler

  • 货号: TS174773
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： Ankistrodesmus braunii Brunnthaler
商品货号： TS174773
Deposited As： Ankistrodesmus falcatus var. duplex
Strain Designations： UTEX 187 CCAP-202/9a
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Material collected by T. Christensen, Madingley Brick Pits, England, 1949
Product Format： frozen
Storage Conditions： Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain： no
Comments：
photosynthetic
Medium： ATCC® Medium 847: Algal proteose agar
Growth Conditions：
Temperature: 25°C
Culture System: Axenic
Cryopreservation： Harvest and Preservation
  1. Harvest cells from a culture that is at or near peak density by centrifugation at 700-800 x g for 5 min.
  2. Adjust the concentration of cells to 2 x 106 - 2 x 107/mL in fresh medium.
  3. While cells are centrifuging prepare a 14% (v/v) solution of sterile DMSO in fresh medium.
  4. Mix the cell preparation and the 14% DMSO solution in equal portions. Thus, the final concentration will be 106 - 107 cells/mL and 7% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution to the beginning of the freezing process should be no less than 5 min and no greater than 15 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the vials in a controlled rate freezing unit.xa0 From room temperature cool at -1°C/min to -40°C.xa0 If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion.xa0 At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0
  7. The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C.
  8. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial.
  9. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and add to a 16 x 125 mm screw-capped test tube containing 5 mL of ATCC medium 847 broth or to the surface of an ATCC medium 847 agar plate (20 x 100 mm Petri plate containing 20 mL of ATCC medium 5 agar).
  10. Incubate the culture at 50-100 µEinsteins/m2/s irradiance at 25°C.xa0 Maintain under a 14/10h light-dark photoperiod.
Name of Depositor： UTEX
Chain of Custody：
ATCC <-- UTEX <-- E A George
Year of Origin： 1949
References：

Starr RC. The culture collection of algae at Indiana University. Am. J. Bot. 51: 1013-1044, 1964.

testopic
小红书
扫码关注小红书账号
关注我们
联系电话：0574-87917803
章丽敏：17757489010
朱静：18067106081
李晴：18067408036
李晶晶：13375187189
周雪：13372109301
邮箱：testobio@163.com
地址：浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
在线留言
合作单位: