| 产品名称： | pHSG-cre |
|---|---|
| 商品货号： | TS175108 |
| Designations： | pHSG-cre |
| Depositors： | ML Pall |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli JM109 |
| Vector Information： | Size (kb): 4.0000000000000000 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pHSG298 Intact vector size: 2.676 Type of vector: plasmid Vector end: EcoRI Vector end: SalI Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI SalI PstI SphI Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI SalI PstI SphI HindIII Other unique sites: ClaI Construction: pHSG1339 Host range: Escherichia coli Features (with orientation and position when available): marker(s): kanR, -> insert detection: lacZ, MCS: HindIII...EcoRI, -> replicon: pSC101 Cross references: Vector: pHSG-cre (plasmid) Promoters: Promoter lac Construction: pHSG298, lambdaKC Marker(s):kanR Construct size (kb): 4.0 Features: marker(s): kanR other: cre promoter: lac replicon: pMB1 |
| Applications： | produces protein recombinase, site-specific vector for other uses |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--2.6, 1.4; EcoRI--4.0; PstI--2.6, 1.4. The cre gene contains the following restriction sites (approximate kb from the 5 end): BamHI--0.84; ClaI--1.29. The lambda/plasmid hybrid vectors can then be used for automatic subcloning; that is, the cre-mediated excision of plasmid constructs. A plasmid useful for generating novel lambda/plasmid hybrid vectors by using cre-lox recombination to exchange new plasmids for plasmids already in lambda/plasmid hybrids. Constructed by ligating a 1.3 kb cre-containing fragment from lambdaKC to pHSG298 digested with EcoRI+SalI. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010 Brunelli JP, Pall ML. Lambda/Plasmid vector construction by in vivo cre/lox-mediated recombination. BioTechniques 16: 1060-1064, 1994. PubMed: 8074871 Takeshita S, et al. High-copy-number and low-copy-number plasmid vectors for lacZalpha-complementation and chloramphenicol- or kanamycin-resistance selection. Gene 61: 63-74, 1987. PubMed: 3327753 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |
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| 产品名称： | pHSG-cre |
|---|---|
| 商品货号： | TS175108 |
| Designations： | pHSG-cre |
| Depositors： | ML Pall |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli JM109 |
| Vector Information： | Size (kb): 4.0000000000000000 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pHSG298 Intact vector size: 2.676 Type of vector: plasmid Vector end: EcoRI Vector end: SalI Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI SalI PstI SphI Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI SalI PstI SphI HindIII Other unique sites: ClaI Construction: pHSG1339 Host range: Escherichia coli Features (with orientation and position when available): marker(s): kanR, -> insert detection: lacZ, MCS: HindIII...EcoRI, -> replicon: pSC101 Cross references: Vector: pHSG-cre (plasmid) Promoters: Promoter lac Construction: pHSG298, lambdaKC Marker(s):kanR Construct size (kb): 4.0 Features: marker(s): kanR other: cre promoter: lac replicon: pMB1 |
| Applications： | produces protein recombinase, site-specific vector for other uses |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--2.6, 1.4; EcoRI--4.0; PstI--2.6, 1.4. The cre gene contains the following restriction sites (approximate kb from the 5 end): BamHI--0.84; ClaI--1.29. The lambda/plasmid hybrid vectors can then be used for automatic subcloning; that is, the cre-mediated excision of plasmid constructs. A plasmid useful for generating novel lambda/plasmid hybrid vectors by using cre-lox recombination to exchange new plasmids for plasmids already in lambda/plasmid hybrids. Constructed by ligating a 1.3 kb cre-containing fragment from lambdaKC to pHSG298 digested with EcoRI+SalI. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010 Brunelli JP, Pall ML. Lambda/Plasmid vector construction by in vivo cre/lox-mediated recombination. BioTechniques 16: 1060-1064, 1994. PubMed: 8074871 Takeshita S, et al. High-copy-number and low-copy-number plasmid vectors for lacZalpha-complementation and chloramphenicol- or kanamycin-resistance selection. Gene 61: 63-74, 1987. PubMed: 3327753 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |
