| 产品名称： | pSREBP-2 |
|---|---|
| 商品货号： | TS175118 |
| Designations： | pSREBP-2 |
| GenBank Number： | U02031 U12330 |
| Species： | Homo sapiens, human |
| Depositors： | JL Goldstein |
| Vector： | Construct size (kb): 8.199999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pEXlox(+) Intact vector size: 4.000 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR replicon: pMB1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: SREBP2 Gene name: sterol regulatory element binding transcription factor 2 Contains complete coding sequence?: U Type of DNA: cDNA Insert end: EcoRI Insert end: EcoRI Insert size (kb): 4.249 Cross references: DNA Seq. Acc.: U02031 Nucleotides 1-4249 of the insert correspond to nucleotides 1-4249 of U02031. Insert lengths(kb): 4.249000072479248 Gene product: sterol regulatory element binding transcription factor 2(sterol regulatory element-binding protein 2) SREBP2 |
| Insert Size (kb)： | 4.249 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.2, 3.0; EcoRV--8.2; SalI--4.25, 3.95; EcoRI--3.95, 2.6, 1.65; EcoRI/SalI--3.95, 2.6, 1.65. The insert contains the following restriction sites (bp from the 5 end): EcoRI--2614; EcoRV--1142; BamHI--2170. The fragment excised by a SalI digest contains 6 bp of adaptor sequence. |
| References： | Hua X, et al. SREBP-2, a second basic-helix-loop-helix-leucine zipper protein that stimulates transcription by binding to a sterol regulatory element. Proc. Natl. Acad. Sci. USA : 11603-11607, 1993. PubMed: 7903453 Hua X, et al. Structure of the human gene encoding sterol regulatory element binding protein-1 (SREBF1) and localization of SREBF1 and SREBF2 to chromsosomes 17p11.2 and 22q13. Genomics 25: 667-673, 1995. PubMed: 7759101 Joseph L Goldstein, personal communication |
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| 产品名称： | pSREBP-2 |
|---|---|
| 商品货号： | TS175118 |
| Designations： | pSREBP-2 |
| GenBank Number： | U02031 U12330 |
| Species： | Homo sapiens, human |
| Depositors： | JL Goldstein |
| Vector： | Construct size (kb): 8.199999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pEXlox(+) Intact vector size: 4.000 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR replicon: pMB1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: SREBP2 Gene name: sterol regulatory element binding transcription factor 2 Contains complete coding sequence?: U Type of DNA: cDNA Insert end: EcoRI Insert end: EcoRI Insert size (kb): 4.249 Cross references: DNA Seq. Acc.: U02031 Nucleotides 1-4249 of the insert correspond to nucleotides 1-4249 of U02031. Insert lengths(kb): 4.249000072479248 Gene product: sterol regulatory element binding transcription factor 2(sterol regulatory element-binding protein 2) SREBP2 |
| Insert Size (kb)： | 4.249 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.2, 3.0; EcoRV--8.2; SalI--4.25, 3.95; EcoRI--3.95, 2.6, 1.65; EcoRI/SalI--3.95, 2.6, 1.65. The insert contains the following restriction sites (bp from the 5 end): EcoRI--2614; EcoRV--1142; BamHI--2170. The fragment excised by a SalI digest contains 6 bp of adaptor sequence. |
| References： | Hua X, et al. SREBP-2, a second basic-helix-loop-helix-leucine zipper protein that stimulates transcription by binding to a sterol regulatory element. Proc. Natl. Acad. Sci. USA : 11603-11607, 1993. PubMed: 7903453 Hua X, et al. Structure of the human gene encoding sterol regulatory element binding protein-1 (SREBF1) and localization of SREBF1 and SREBF2 to chromsosomes 17p11.2 and 22q13. Genomics 25: 667-673, 1995. PubMed: 7759101 Joseph L Goldstein, personal communication |
