| 产品名称： | Blastocrithidia culicis (Novy et al.) Wallace and Johnson |
|---|---|
| 商品货号： | TS175219 |
| Strain Designations： | adiplosomic |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | mosquito, Aedes vexans, Mound, MN, 1952 |
| Product Format： | frozen |
| Type Strain： | no |
| Comments： | nutritional requirements Urea cycle in wild aposymbiotic strains isoenzyme electrophoresis for species identification Trypomastigotes in cultures |
| Medium： | ATCC® Medium 1011: Diphasic blood agar medium |
| Growth Conditions： | Max Temperature: 25.0°C Min Temperature: 21.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. |
| Subcultivation： | Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. |
| Name of Depositor： | FG Wallace |
| Year of Origin： | 1952 |
| References： | De Menezes MC, Roitman I. Nutritional requirements of Blastocrithidia culicis, a trypanosomatid with an endosymbiont. J. Protozool. 38: 122-123, 1991. Wallace and Johnson. . J. Insect Pathol. 3: 75-80, 1961. Eberhard ML, et al. Development of Dipetalonema gracile and D. caudispina to the infective stage in Culicoides hollensis. J. Parasitol. 65: 89-95, 1979. PubMed: 571910 Goncalves de Lima VM, et al. Five trypanosomatid species of insects distinguished by isoenzymes. J. Protozool. 26: 648-652, 1979. PubMed: 161788 de Sousa MA. Trypomastigotes in cultures of Blastocrithidia culicis (Novy, MacNeal & Torrey, 1907) (Kinetoplastida: Trypanosomatidae). Mem. Inst. Oswaldo Cruz Rio J. 89: 111-112, 1994. PubMed: 7823806 Camargo EP, et al. Electrophoretic analysis of endonuclease-generated fragments of k-DNA, of esterase isoenzymes, and of surface proteins as aids for species identification of insect trypanosomatids. J. Protozool. 29: 251-258, 1982. PubMed: 6284925 |
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| 产品名称： | Blastocrithidia culicis (Novy et al.) Wallace and Johnson |
|---|---|
| 商品货号： | TS175219 |
| Strain Designations： | adiplosomic |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | mosquito, Aedes vexans, Mound, MN, 1952 |
| Product Format： | frozen |
| Type Strain： | no |
| Comments： | nutritional requirements Urea cycle in wild aposymbiotic strains isoenzyme electrophoresis for species identification Trypomastigotes in cultures |
| Medium： | ATCC® Medium 1011: Diphasic blood agar medium |
| Growth Conditions： | Max Temperature: 25.0°C Min Temperature: 21.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. |
| Subcultivation： | Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. |
| Name of Depositor： | FG Wallace |
| Year of Origin： | 1952 |
| References： | De Menezes MC, Roitman I. Nutritional requirements of Blastocrithidia culicis, a trypanosomatid with an endosymbiont. J. Protozool. 38: 122-123, 1991. Wallace and Johnson. . J. Insect Pathol. 3: 75-80, 1961. Eberhard ML, et al. Development of Dipetalonema gracile and D. caudispina to the infective stage in Culicoides hollensis. J. Parasitol. 65: 89-95, 1979. PubMed: 571910 Goncalves de Lima VM, et al. Five trypanosomatid species of insects distinguished by isoenzymes. J. Protozool. 26: 648-652, 1979. PubMed: 161788 de Sousa MA. Trypomastigotes in cultures of Blastocrithidia culicis (Novy, MacNeal & Torrey, 1907) (Kinetoplastida: Trypanosomatidae). Mem. Inst. Oswaldo Cruz Rio J. 89: 111-112, 1994. PubMed: 7823806 Camargo EP, et al. Electrophoretic analysis of endonuclease-generated fragments of k-DNA, of esterase isoenzymes, and of surface proteins as aids for species identification of insect trypanosomatids. J. Protozool. 29: 251-258, 1982. PubMed: 6284925 |
