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pFM24
pFM24
规格:
货期:
编号:TS175269
品牌:Testobio
产品名称: pFM24
商品货号: TS175269
Designations: pFM24
GenBank Number:

M33002

Species: tobacco necrosis necrovirus
Depositors: F Meulewaeter
Vector:
Construct size (kb): 4.360000133514404
Insert:
DNA: cDNA
Insert lengths(kb): 1.613999962806702
Gene product: ORF1, 23 kDa ORF1
Insert Size (kb): 1.614
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--2.8, 1.7; XhoI--4.4; KpnI--3.1, 1.25.
The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.15; BglII--0.16; SpeI--0.97; KpnI--1.18; XhoI--1.37.
The plasmid is not stable in E. coli MC1061.
Includes nt 19-1632 of the virus, including the complete ORF1 and part of ORF2.
Transcription from the T7 promoter gives the sense (+) strand.
Classification: Necrovirus
References:

Meulewaeter F, et al. Genome structure of tobacco necrosis virus strain A. Virology 177: 699-709, 1990. PubMed: 2371773

Frank Meulewaeter, personal communication

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pFM24

  • 货号: TS175269
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  • 品牌 : TESTOBIO
产品名称: pFM24
商品货号: TS175269
Designations: pFM24
GenBank Number:

M33002

Species: tobacco necrosis necrovirus
Depositors: F Meulewaeter
Vector:
Construct size (kb): 4.360000133514404
Insert:
DNA: cDNA
Insert lengths(kb): 1.613999962806702
Gene product: ORF1, 23 kDa ORF1
Insert Size (kb): 1.614
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--2.8, 1.7; XhoI--4.4; KpnI--3.1, 1.25.
The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.15; BglII--0.16; SpeI--0.97; KpnI--1.18; XhoI--1.37.
The plasmid is not stable in E. coli MC1061.
Includes nt 19-1632 of the virus, including the complete ORF1 and part of ORF2.
Transcription from the T7 promoter gives the sense (+) strand.
Classification: Necrovirus
References:

Meulewaeter F, et al. Genome structure of tobacco necrosis virus strain A. Virology 177: 699-709, 1990. PubMed: 2371773

Frank Meulewaeter, personal communication

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