| 产品名称： | TC870 |
|---|---|
| 商品货号： | TS175911 |
| Designations： | TC870 |
| GenBank Number： | J03561 |
| Species： | Homo sapiens, human |
| Depositors： | A Anisowicz |
| Vector： | Construct size (kb): 3.710000038146973 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.8399999737739563 Tissue: bladder carcinoma T24 cell line Gene product: GRO1 oncogene (melanoma growth stimulating activity, alpha)(growth regulated (alpha), growth regulated (group I)) GRO Alleles: A1, A2, A1, A1, A2, A2, A1, A2 |
| Insert Size (kb)： | 0.840 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 0.8; HindIII--3.5, 0.2; BamHI--3.1, 0.6; PstI--3.4, 0.3; PvuII--3.1, 0.6. Contains the EcoRI fragment corresponding to nt 205 to nt 1050 of the published sequence. The insert includes the following restriction sites in nt from the 5 end: HindIII--96; PvuII--257, PstI--262; BamHI--568. The insert is inserted such that transcription from the SP6 promoter gives the sense strand and from the T7 promoter gives the antisense strand. Enzyme(s) not detecting polymorphism: AluI, AlwNI, ApaI, ApaLI, AseI, AvaI, BamHI, BanII, BclI, BglI, BglII, BspHI, BstUI, DraI, Eco0109, EcoRI, HaeIII, HincII, HindIII, HphI, KpnI, MspI, NraI, PstI, PvuII, RsaI, SacI, Sau3AI, SmaI, StuI, TaqI, XbaI. |
| References： | Beck JS, et al. A ScaI RFLP demonstrated for the GRO gene on chromosome 4. Nucleic Acids Res. 17: 8895, 1989. PubMed: 2573881 Haskill S, et al. Identification of three related human GRO genes encoding cytokine functions. Proc. Natl. Acad. Sci. USA 87: 7732-7736, 1990. PubMed: 2217207 Anisowicz A, et al. Constitutive overexpression of a growth-regulated gene in transformed Chinese hamster and human cells. Proc. Natl. Acad. Sci. USA 84: 7188-7192, 1987. PubMed: 2890161 Anthony Anisowicz, personal communication |
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| 产品名称： | TC870 |
|---|---|
| 商品货号： | TS175911 |
| Designations： | TC870 |
| GenBank Number： | J03561 |
| Species： | Homo sapiens, human |
| Depositors： | A Anisowicz |
| Vector： | Construct size (kb): 3.710000038146973 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.8399999737739563 Tissue: bladder carcinoma T24 cell line Gene product: GRO1 oncogene (melanoma growth stimulating activity, alpha)(growth regulated (alpha), growth regulated (group I)) GRO Alleles: A1, A2, A1, A1, A2, A2, A1, A2 |
| Insert Size (kb)： | 0.840 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 0.8; HindIII--3.5, 0.2; BamHI--3.1, 0.6; PstI--3.4, 0.3; PvuII--3.1, 0.6. Contains the EcoRI fragment corresponding to nt 205 to nt 1050 of the published sequence. The insert includes the following restriction sites in nt from the 5 end: HindIII--96; PvuII--257, PstI--262; BamHI--568. The insert is inserted such that transcription from the SP6 promoter gives the sense strand and from the T7 promoter gives the antisense strand. Enzyme(s) not detecting polymorphism: AluI, AlwNI, ApaI, ApaLI, AseI, AvaI, BamHI, BanII, BclI, BglI, BglII, BspHI, BstUI, DraI, Eco0109, EcoRI, HaeIII, HincII, HindIII, HphI, KpnI, MspI, NraI, PstI, PvuII, RsaI, SacI, Sau3AI, SmaI, StuI, TaqI, XbaI. |
| References： | Beck JS, et al. A ScaI RFLP demonstrated for the GRO gene on chromosome 4. Nucleic Acids Res. 17: 8895, 1989. PubMed: 2573881 Haskill S, et al. Identification of three related human GRO genes encoding cytokine functions. Proc. Natl. Acad. Sci. USA 87: 7732-7736, 1990. PubMed: 2217207 Anisowicz A, et al. Constitutive overexpression of a growth-regulated gene in transformed Chinese hamster and human cells. Proc. Natl. Acad. Sci. USA 84: 7188-7192, 1987. PubMed: 2890161 Anthony Anisowicz, personal communication |
